BRENDA - Enzyme Database
show all sequences of 3.4.23.40

Structure and possible function of aspartic proteinases in barley and other plants

Kervinen, J.; Törmäkangas, K.; Runeberg-Roos, P.; Guruprasad, K.; Blundell, T.; Teeri, T.H.; Adv. Exp. Med. Biol. 362, 241-254 (1995) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Triticum aestivum
Diazoacetylnorleucine methyl ester
-
Triticum aestivum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
vacuole
-
Hordeum vulgare
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
seeds, gel filtration
Triticum aestivum
89000
-
leaf, gel filtration
Triticum aestivum
66000
-
gel filtration
Triticum durum x Haynaldia villosa
58000
-
1 * 58000, SDS-PAGE
Triticum aestivum
59000
-
gel filtration
Nepenthes macfarlanei
20000
-
ultracentirfugation
Cannabis sativa
40000
-
2 * 40000, sequence of cDNA
Hordeum vulgare
37000
-
1 * 37000, SDS-PAGE
Solanum lycopersicum
45000
-
-
Theobroma cacao
48000
-
calculation from sequence of cDNA
Oryza sativa
48000
-
2 * 48000, SDS-PAGE
Hordeum vulgare
34000
-
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
28000
-
gel filtration
Fagopyrum esculentum
36000
37000
ultracentrifugation, gel filtration
Nelumbo nucifera
36000
40000
gel filtration
Nicotiana tabacum
40000
42000
gel filtration
Cucumis sativus
40000
42000
gel filtration
Cucurbita maxima
60000
65000
gel filtration
Oryza sativa
16000
-
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Protein + H2O
Triticum aestivum
-
?
-
-
?
Protein + H2O
Brassica napus
-
?
-
-
?
Protein + H2O
Cucumis sativus
-
?
-
-
?
Protein + H2O
Pinus sylvestris
-
?
-
-
?
Protein + H2O
Sorghum bicolor
-
?
-
-
?
Protein + H2O
Oryza sativa
-
?
-
-
?
Protein + H2O
Cucurbita maxima
-
?
-
-
?
Protein + H2O
Fagopyrum esculentum
-
?
-
-
?
Protein + H2O
Pinus banksiana
-
?
-
-
?
Protein + H2O
Triticum durum x Haynaldia villosa
-
?
-
-
?
Protein + H2O
Cannabis sativa
-
?
-
-
?
Protein + H2O
Nelumbo nucifera
-
?
-
-
?
Protein + H2O
Cynara cardunculus
-
?
-
-
?
Protein + H2O
Theobroma cacao
-
?
-
-
?
Protein + H2O
Drosera peltata
-
?
-
-
?
Protein + H2O
Hordeum vulgare
may participate in the processing of the C-terminus of barley prolectin
?
-
-
?
Protein + H2O
Nicotiana tabacum
hydrolysis of pathogenesis-related proteins
?
-
-
?
Protein + H2O
Solanum lycopersicum
hydrolysis of pathogenesis-related proteins
?
-
-
?
Protein + H2O
Nepenthes macfarlanei
contribution to the digestion of trapped insects
?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Brassica napus
-
rape
-
Cannabis sativa
-
hemp
-
Cucumis sativus
-
cucumber
-
Cucurbita maxima
-
squash
-
Cynara cardunculus
-
cardoon
-
Drosera peltata
-
-
-
Fagopyrum esculentum
-
buckwheat
-
Hordeum vulgare
-
L. Cv. Kustaa, barley
-
Solanum lycopersicum
-
tomato
-
Nelumbo nucifera
-
lotus
-
Nepenthes macfarlanei
-
-
-
Nicotiana tabacum
-
tobacco
-
Oryza sativa
-
rice
-
Pinus banksiana
-
Jack pine
-
Pinus sylvestris
-
Scots pine
-
Sorghum bicolor
-
sorghum
-
Theobroma cacao
-
cacao
-
Triticum aestivum
-
wheat
-
Triticum durum x Haynaldia villosa
-
hybrid between Triticum durum and Hynaldia villosa
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Nelumbo nucifera
-
seed
-
Triticum aestivum
-
seed
-
Brassica napus
-
seed
-
Cucumis sativus
-
seed
-
Pinus sylvestris
-
seed
-
Sorghum bicolor
-
seed
-
Cucurbita maxima
-
seed
-
Fagopyrum esculentum
-
seed
-
Pinus banksiana
-
seed
-
Triticum durum x Haynaldia villosa
-
seed
-
Cannabis sativa
-
seed
-
Nelumbo nucifera
-
seed
-
Theobroma cacao
-
seed
embryo, aleurone layer
Oryza sativa
-
seed
developing, resting and germinating grains, in the embryo, aleurone layer, testa and pericarp
Hordeum vulgare
-
leaf
-
Triticum aestivum
-
leaf
-
Hordeum vulgare
-
leaf
-
Nicotiana tabacum
-
leaf
-
Solanum lycopersicum
-
stem
-
Drosera peltata
-
flower
-
Cynara cardunculus
-
pitcher secretion
-
Nepenthes macfarlanei
-
trapping leaf
-
Drosera peltata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Protein + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Protein + H2O
-
30844
Brassica napus
?
-
-
-
?
Protein + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Protein + H2O
-
30844
Pinus sylvestris
?
-
-
-
?
Protein + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Protein + H2O
-
30844
Oryza sativa
?
-
-
-
?
Protein + H2O
-
30844
Cucurbita maxima
?
-
-
-
?
Protein + H2O
-
30844
Fagopyrum esculentum
?
-
-
-
?
Protein + H2O
-
30844
Pinus banksiana
?
-
-
-
?
Protein + H2O
-
30844
Triticum durum x Haynaldia villosa
?
-
-
-
?
Protein + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Protein + H2O
-
30844
Nelumbo nucifera
?
-
-
-
?
Protein + H2O
-
30844
Cynara cardunculus
?
-
-
-
?
Protein + H2O
-
30844
Theobroma cacao
?
-
-
-
?
Protein + H2O
-
30844
Drosera peltata
?
-
-
-
?
Protein + H2O
may participate in the processing of the C-terminus of barley prolectin
30844
Hordeum vulgare
?
-
-
-
?
Protein + H2O
hydrolysis of pathogenesis-related proteins
30844
Nicotiana tabacum
?
-
-
-
?
Protein + H2O
hydrolysis of pathogenesis-related proteins
30844
Solanum lycopersicum
?
-
-
-
?
Protein + H2O
contribution to the digestion of trapped insects
30844
Nepenthes macfarlanei
?
-
-
-
?
casein + H2O
-
30844
Brassica napus
?
-
-
-
?
casein + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Hemoglobin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Nicotiana tabacum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Solanum lycopersicum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Hemoglobin + H2O
-
30844
Pinus sylvestris
?
-
-
-
?
Hemoglobin + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cucurbita maxima
?
-
-
-
?
Hemoglobin + H2O
-
30844
Pinus banksiana
?
-
-
-
?
Hemoglobin + H2O
-
30844
Triticum durum x Haynaldia villosa
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Hemoglobin + H2O
-
30844
Nelumbo nucifera
?
-
-
-
?
Albumin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Albumin + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Albumin + H2O
-
30844
Fagopyrum esculentum
?
-
-
-
?
Albumin + H2O
-
30844
Nepenthes macfarlanei
?
-
-
-
?
Albumin + H2O
-
30844
Theobroma cacao
?
-
-
-
?
Gliadin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Edestin + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Fibrin + H2O
-
30844
Nepenthes macfarlanei
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Hordeum vulgare
dimer
2 * 40000, sequence of cDNA
Hordeum vulgare
dimer
2 * 46000-49000, SDS-PAGE, sequence of cDNA
Cynara cardunculus
dimer
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
monomer
1 * 58000, SDS-PAGE
Triticum aestivum
monomer
1 * 37000, SDS-PAGE
Solanum lycopersicum
Synonyms
Synonyms
Commentary
Organism
Nepenthesin
-
Nepenthes macfarlanei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
substrate hemoglobin
Nicotiana tabacum
3
-
substrate albumin
Triticum aestivum
4.5
-
substrate gliadin
Triticum aestivum
3.5
-
substrate hemoglobin
Pinus banksiana
3.5
-
substrate albumin
Brassica napus
3.5
-
substrate albumin
Fagopyrum esculentum
3.2
-
substrate hemoglobin
Cucumis sativus
3.4
-
substrate hemoglobin
Cannabis sativa
3.4
-
substrate albumin
Theobroma cacao
4.3
-
substrate edestin
Cannabis sativa
5.1
-
substrate casein
Cynara cardunculus
3.6
-
substrate hemoglobin
Cucurbita maxima
3.6
-
substrate albumin
Sorghum bicolor
2.5
3.5
substrate hemoglobin
Solanum lycopersicum
3.7
-
substrate hemoglobin
Pinus sylvestris
2.2
-
substrates albumin, fibrin
Nepenthes macfarlanei
2.4
3.8
substrates casein, hemoglobin
Nelumbo nucifera
2.5
4.2
substrates casein, hemoglobin
Oryza sativa
2
3
substrate casein
Drosera peltata
3.1
-
chromophoric peptide substrate
Triticum durum x Haynaldia villosa
3.5
3.9
substrate hemoglobin
Hordeum vulgare
3.7
4.1
chromophoric peptide substrate
Hordeum vulgare
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Triticum aestivum
Diazoacetylnorleucine methyl ester
-
Triticum aestivum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
vacuole
-
Hordeum vulgare
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
seeds, gel filtration
Triticum aestivum
89000
-
leaf, gel filtration
Triticum aestivum
66000
-
gel filtration
Triticum durum x Haynaldia villosa
58000
-
1 * 58000, SDS-PAGE
Triticum aestivum
59000
-
gel filtration
Nepenthes macfarlanei
20000
-
ultracentirfugation
Cannabis sativa
40000
-
2 * 40000, sequence of cDNA
Hordeum vulgare
37000
-
1 * 37000, SDS-PAGE
Solanum lycopersicum
45000
-
-
Theobroma cacao
48000
-
calculation from sequence of cDNA
Oryza sativa
48000
-
2 * 48000, SDS-PAGE
Hordeum vulgare
34000
-
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
28000
-
gel filtration
Fagopyrum esculentum
36000
37000
ultracentrifugation, gel filtration
Nelumbo nucifera
36000
40000
gel filtration
Nicotiana tabacum
40000
42000
gel filtration
Cucumis sativus
40000
42000
gel filtration
Cucurbita maxima
60000
65000
gel filtration
Oryza sativa
16000
-
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Protein + H2O
Triticum aestivum
-
?
-
-
?
Protein + H2O
Brassica napus
-
?
-
-
?
Protein + H2O
Cucumis sativus
-
?
-
-
?
Protein + H2O
Pinus sylvestris
-
?
-
-
?
Protein + H2O
Sorghum bicolor
-
?
-
-
?
Protein + H2O
Oryza sativa
-
?
-
-
?
Protein + H2O
Cucurbita maxima
-
?
-
-
?
Protein + H2O
Fagopyrum esculentum
-
?
-
-
?
Protein + H2O
Pinus banksiana
-
?
-
-
?
Protein + H2O
Triticum durum x Haynaldia villosa
-
?
-
-
?
Protein + H2O
Cannabis sativa
-
?
-
-
?
Protein + H2O
Nelumbo nucifera
-
?
-
-
?
Protein + H2O
Cynara cardunculus
-
?
-
-
?
Protein + H2O
Theobroma cacao
-
?
-
-
?
Protein + H2O
Drosera peltata
-
?
-
-
?
Protein + H2O
Hordeum vulgare
may participate in the processing of the C-terminus of barley prolectin
?
-
-
?
Protein + H2O
Nicotiana tabacum
hydrolysis of pathogenesis-related proteins
?
-
-
?
Protein + H2O
Solanum lycopersicum
hydrolysis of pathogenesis-related proteins
?
-
-
?
Protein + H2O
Nepenthes macfarlanei
contribution to the digestion of trapped insects
?
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Nelumbo nucifera
-
seed
-
Triticum aestivum
-
seed
-
Brassica napus
-
seed
-
Cucumis sativus
-
seed
-
Pinus sylvestris
-
seed
-
Sorghum bicolor
-
seed
-
Cucurbita maxima
-
seed
-
Fagopyrum esculentum
-
seed
-
Pinus banksiana
-
seed
-
Triticum durum x Haynaldia villosa
-
seed
-
Cannabis sativa
-
seed
-
Nelumbo nucifera
-
seed
-
Theobroma cacao
-
seed
embryo, aleurone layer
Oryza sativa
-
seed
developing, resting and germinating grains, in the embryo, aleurone layer, testa and pericarp
Hordeum vulgare
-
leaf
-
Triticum aestivum
-
leaf
-
Hordeum vulgare
-
leaf
-
Nicotiana tabacum
-
leaf
-
Solanum lycopersicum
-
stem
-
Drosera peltata
-
flower
-
Cynara cardunculus
-
pitcher secretion
-
Nepenthes macfarlanei
-
trapping leaf
-
Drosera peltata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Protein + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Protein + H2O
-
30844
Brassica napus
?
-
-
-
?
Protein + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Protein + H2O
-
30844
Pinus sylvestris
?
-
-
-
?
Protein + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Protein + H2O
-
30844
Oryza sativa
?
-
-
-
?
Protein + H2O
-
30844
Cucurbita maxima
?
-
-
-
?
Protein + H2O
-
30844
Fagopyrum esculentum
?
-
-
-
?
Protein + H2O
-
30844
Pinus banksiana
?
-
-
-
?
Protein + H2O
-
30844
Triticum durum x Haynaldia villosa
?
-
-
-
?
Protein + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Protein + H2O
-
30844
Nelumbo nucifera
?
-
-
-
?
Protein + H2O
-
30844
Cynara cardunculus
?
-
-
-
?
Protein + H2O
-
30844
Theobroma cacao
?
-
-
-
?
Protein + H2O
-
30844
Drosera peltata
?
-
-
-
?
Protein + H2O
may participate in the processing of the C-terminus of barley prolectin
30844
Hordeum vulgare
?
-
-
-
?
Protein + H2O
hydrolysis of pathogenesis-related proteins
30844
Nicotiana tabacum
?
-
-
-
?
Protein + H2O
hydrolysis of pathogenesis-related proteins
30844
Solanum lycopersicum
?
-
-
-
?
Protein + H2O
contribution to the digestion of trapped insects
30844
Nepenthes macfarlanei
?
-
-
-
?
casein + H2O
-
30844
Brassica napus
?
-
-
-
?
casein + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Hemoglobin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Nicotiana tabacum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Solanum lycopersicum
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cucumis sativus
?
-
-
-
?
Hemoglobin + H2O
-
30844
Pinus sylvestris
?
-
-
-
?
Hemoglobin + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cucurbita maxima
?
-
-
-
?
Hemoglobin + H2O
-
30844
Pinus banksiana
?
-
-
-
?
Hemoglobin + H2O
-
30844
Triticum durum x Haynaldia villosa
?
-
-
-
?
Hemoglobin + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Hemoglobin + H2O
-
30844
Nelumbo nucifera
?
-
-
-
?
Albumin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Albumin + H2O
-
30844
Sorghum bicolor
?
-
-
-
?
Albumin + H2O
-
30844
Fagopyrum esculentum
?
-
-
-
?
Albumin + H2O
-
30844
Nepenthes macfarlanei
?
-
-
-
?
Albumin + H2O
-
30844
Theobroma cacao
?
-
-
-
?
Gliadin + H2O
-
30844
Triticum aestivum
?
-
-
-
?
Edestin + H2O
-
30844
Cannabis sativa
?
-
-
-
?
Fibrin + H2O
-
30844
Nepenthes macfarlanei
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 48000, SDS-PAGE
Hordeum vulgare
dimer
2 * 40000, sequence of cDNA
Hordeum vulgare
dimer
2 * 46000-49000, SDS-PAGE, sequence of cDNA
Cynara cardunculus
dimer
1 * 34000 + 1 * 16000, SDS-PAGE
Theobroma cacao
monomer
1 * 58000, SDS-PAGE
Triticum aestivum
monomer
1 * 37000, SDS-PAGE
Solanum lycopersicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3
-
substrate hemoglobin
Nicotiana tabacum
3
-
substrate albumin
Triticum aestivum
4.5
-
substrate gliadin
Triticum aestivum
3.5
-
substrate hemoglobin
Pinus banksiana
3.5
-
substrate albumin
Brassica napus
3.5
-
substrate albumin
Fagopyrum esculentum
3.2
-
substrate hemoglobin
Cucumis sativus
3.4
-
substrate hemoglobin
Cannabis sativa
3.4
-
substrate albumin
Theobroma cacao
4.3
-
substrate edestin
Cannabis sativa
5.1
-
substrate casein
Cynara cardunculus
3.6
-
substrate hemoglobin
Cucurbita maxima
3.6
-
substrate albumin
Sorghum bicolor
2.5
3.5
substrate hemoglobin
Solanum lycopersicum
3.7
-
substrate hemoglobin
Pinus sylvestris
2.2
-
substrates albumin, fibrin
Nepenthes macfarlanei
2.4
3.8
substrates casein, hemoglobin
Nelumbo nucifera
2.5
4.2
substrates casein, hemoglobin
Oryza sativa
2
3
substrate casein
Drosera peltata
3.1
-
chromophoric peptide substrate
Triticum durum x Haynaldia villosa
3.5
3.9
substrate hemoglobin
Hordeum vulgare
3.7
4.1
chromophoric peptide substrate
Hordeum vulgare
Other publictions for EC 3.4.23.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755355
Wu
Cardosin A endocytosis mediat ...
Cynara cardunculus
Proteins
87
502-511
2019
-
-
-
-
-
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-
-
-
-
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-
-
10
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2
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1
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
752567
Almeida
Functional and structural cha ...
Cynara cardunculus
Appl. Microbiol. Biotechnol.
101
6951-6968
2017
-
-
1
-
-
-
2
3
-
-
-
-
-
5
-
1
-
-
-
1
-
-
4
1
3
1
1
-
3
1
1
-
-
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-
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-
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1
-
-
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-
-
2
-
3
-
-
-
-
-
-
1
-
-
1
-
-
4
1
1
1
-
3
1
1
-
-
-
-
-
-
3
3
733194
Almeida
Engineering a cardosin B-deriv ...
Cynara cardunculus
Appl. Microbiol. Biotechnol.
99
269-281
2015
-
1
1
-
-
-
-
-
-
-
2
-
-
12
-
1
-
-
-
3
-
-
3
1
2
1
-
2
-
1
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-
-
-
-
1
1
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-
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-
-
-
-
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-
2
-
-
-
1
-
-
3
-
-
3
1
1
-
2
-
1
-
-
-
1
-
-
1
-
-
733901
Llorente
Use of artichoke (Cynara scoly ...
Cynara cardunculus var. scolymus
Food Chem.
159
55-63
2014
-
1
-
-
-
-
-
-
-
-
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5
-
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1
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1
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-
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-
-
734555
Raimbault
A novel aspartic acid protease ...
Ananas comosus
J. Plant Physiol.
170
1536-1540
2013
-
1
-
-
-
-
-
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1
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4
-
1
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1
1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
1
1
-
-
1
-
-
734915
Pereira
Cardosin A contains two vacuol ...
Cynara cardunculus
Plant J.
76
87-100
2013
-
-
-
-
-
-
-
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1
-
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5
-
1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
669314
Castanheira
Activation, proteolytic proces ...
Cynara cardunculus
J. Biol. Chem.
280
13047-13054
2005
-
-
-
-
1
-
2
1
-
-
4
-
-
4
-
1
-
-
-
1
-
-
2
1
-
1
-
-
1
1
1
-
-
1
-
-
-
-
-
-
-
1
-
-
2
1
1
-
-
4
-
-
-
1
-
-
1
-
-
2
1
1
-
-
1
1
1
-
-
-
-
-
-
-
-
670515
Sidrach
Purification of cynarases from ...
Cynara cardunculus var. scolymus
Phytochemistry
66
41-49
2005
-
-
-
-
-
-
1
1
-
-
8
-
-
8
-
1
1
-
-
1
-
-
1
1
3
1
-
2
1
1
2
-
-
1
3
-
-
-
-
-
-
-
-
-
1
1
1
-
-
8
-
-
-
1
1
-
1
-
-
1
1
1
-
2
1
1
2
-
3
-
-
-
-
-
-
649256
Prasad
Role of water molecules in the ...
Hordeum vulgare
Acta Crystallogr. Sect. D
D58
250-259
2002
-
-
-
1
-
-
-
-
-
-
-
-
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1
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650630
Park
Autoproteolytic processing of ...
Helianthus annuus
Biosci. Biotechnol. Biochem.
65
702-705
2001
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
1
-
-
-
1
-
-
-
1
-
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-
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
3
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
734130
Frazao
Crystal structure of cardosin ...
Cynara cardunculus
J. Biol. Chem.
274
27694-27701
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30849
Belozersky
Aspartic proteinase from wheat ...
Triticum aestivum
Planta
177
321-326
1998
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
2
-
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
-
1
-
1
-
2
-
1
-
-
-
-
-
-
-
733764
Verissimo
Purification, characterization ...
Cynara cardunculus
Eur. J. Biochem.
235
762-768
1996
-
-
-
-
-
-
2
4
-
-
4
-
-
4
-
-
-
-
-
-
1
-
5
2
2
-
-
1
4
1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
4
2
4
-
-
4
-
-
-
-
-
-
-
2
-
6
2
-
-
2
4
2
2
-
-
-
-
-
-
4
4
30844
Kervinen
Structure and possible functio ...
Brassica napus, Cannabis sativa, Cucumis sativus, Cucurbita maxima, Cynara cardunculus, Drosera peltata, Fagopyrum esculentum, Hordeum vulgare, Solanum lycopersicum, Nelumbo nucifera, Nepenthes macfarlanei, Nicotiana tabacum, Oryza sativa, Pinus banksiana, Pinus sylvestris, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Triticum durum x Haynaldia villosa
Adv. Exp. Med. Biol.
362
241-254
1995
-
-
-
-
-
-
2
-
1
-
19
19
-
19
-
-
-
-
-
23
-
-
40
6
1
-
-
-
-
22
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
19
19
-
-
-
-
-
23
-
-
40
6
-
-
-
-
22
-
-
-
-
-
-
-
-
-
30845
Asakura
Rice aspartic proteinase, oryz ...
Oryza sativa
Eur. J. Biochem.
232
77-83
1995
-
-
1
-
-
-
-
-
-
-
-
-
-
24
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30847
Guruprasad
Comparative modelling of barle ...
Hordeum vulgare
FEBS Lett.
352
131-136
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
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1
-
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1
-
-
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-
-
-
-
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-
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-
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30848
Runeberg-Roos
The aspartic proteinase is a v ...
Hordeum vulgare
Plant Physiol.
105
321-329
1994
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
1
-
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-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30851
Törmäkangas
-
Tissue-specific localization o ...
Hordeum vulgare
Planta
195
116-125
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30853
Galleschi
-
Purification, characterization ...
Triticum aestivum
Plant Sci.
98
15-24
1994
-
-
-
-
-
-
1
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
30843
Kervinen
Hydrolytic specificity of barl ...
Hordeum vulgare
Phytochemistry
32
799-803
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
6
-
-
7
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30850
Sarkkinen
Aspartic proteinase from barle ...
Hordeum vulgare
Planta
186
317-323
1992
-
-
-
-
-
-
4
-
-
-
4
-
-
1
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
30846
Runeberg-Roos
Primary structure of a barley- ...
Hordeum vulgare
Eur. J. Biochem.
202
1021-1027
1991
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
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-
-
-
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-
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-
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30852
Galleschi
-
A rapid and sensitive method o ...
Triticum aestivum, Triticum durum x Haynaldia villosa
Plant Physiol. Biochem.
28
793-797
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
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-
-
2
-
-
-
-
-
-
-
-
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-
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-
-
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2
-
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-
2
-
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-
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