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Literature summary for 3.4.23.4 extracted from

  • Ersoez, F.; Inan, M.
    Large-scale production of yak (Bos grunniens) chymosin A in Pichia pastoris (2019), Protein Expr. Purif., 154, 126-133 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Komagataella phaffii (Pichia pastoris strain GS115) Bos grunniens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 40 mM used in milk-clotting assay Bos grunniens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
casein + H2O Bos grunniens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bos grunniens M4LK61
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the preproenzyme has 2 glycosylation sites at N310Q and N349Q Bos grunniens

Source Tissue

Source Tissue Comment Organism Textmining
stomach the enzyme is secreted in the fourth stomach of newborn ruminants Bos grunniens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
Bos grunniens ?
-
?
additional information in acidic conditions, the inactive proenzyme converts to the active form by autocatalytically cleavage of prosequence Bos grunniens ?
-
?
skim milk + H2O
-
Bos grunniens ?
-
?

Subunits

Subunits Comment Organism
? x * 35682, mature enzyme, calculated from amino acid sequence Bos grunniens
? x * 40506, proenzyme, calculated from amino acid sequence Bos grunniens
? x * 42208, preproenzyme, calculated from amino acid sequence Bos grunniens

Synonyms

Synonyms Comment Organism
chymosin A
-
Bos grunniens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bos grunniens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 the enzyme shows about 30% activity at 20°C, about 85% activity at 30°C, 100% activity at 40°C, about 70% activity at 50°C and about 60% activity at 60°C Bos grunniens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 70 after 1 h at 4, 10, 20, 30, 40, 50 and 60°C, the enzyme shows about 99%, 80%, 70%, 60%, 60%, 55% and 40% residual activity, respectively. The enzyme is inactive after 1 h at 70°C Bos grunniens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
Bos grunniens

pH Range

pH Minimum pH Maximum Comment Organism
4 8 the enzyme shows about 60% activity at pH 4.0, about 80% activity at pH 5.5, 100% activity at pH 6.0, about 95% activity at pH 7.0, about 38% activity at pH 7.5, and about 20% activity at pH 8.0 Bos grunniens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7 the enzyme remains stable (100% activity) for 1 h at pH 3.5-7.0. At pH 7.5 and 8.0, the activity drops to about 40% and 20%, respectively. The enzyme is inactive after 1 h at pH 9.0 Bos grunniens