Cloned (Comment) | Organism |
---|---|
expressed in Komagataella phaffii (Pichia pastoris strain GS115) | Bos grunniens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 40 mM used in milk-clotting assay | Bos grunniens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | Bos grunniens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos grunniens | M4LK61 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the preproenzyme has 2 glycosylation sites at N310Q and N349Q | Bos grunniens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | the enzyme is secreted in the fourth stomach of newborn ruminants | Bos grunniens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | - |
Bos grunniens | ? | - |
? | |
additional information | in acidic conditions, the inactive proenzyme converts to the active form by autocatalytically cleavage of prosequence | Bos grunniens | ? | - |
? | |
skim milk + H2O | - |
Bos grunniens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35682, mature enzyme, calculated from amino acid sequence | Bos grunniens |
? | x * 40506, proenzyme, calculated from amino acid sequence | Bos grunniens |
? | x * 42208, preproenzyme, calculated from amino acid sequence | Bos grunniens |
Synonyms | Comment | Organism |
---|---|---|
chymosin A | - |
Bos grunniens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bos grunniens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | the enzyme shows about 30% activity at 20°C, about 85% activity at 30°C, 100% activity at 40°C, about 70% activity at 50°C and about 60% activity at 60°C | Bos grunniens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 70 | after 1 h at 4, 10, 20, 30, 40, 50 and 60°C, the enzyme shows about 99%, 80%, 70%, 60%, 60%, 55% and 40% residual activity, respectively. The enzyme is inactive after 1 h at 70°C | Bos grunniens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Bos grunniens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the enzyme shows about 60% activity at pH 4.0, about 80% activity at pH 5.5, 100% activity at pH 6.0, about 95% activity at pH 7.0, about 38% activity at pH 7.5, and about 20% activity at pH 8.0 | Bos grunniens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | the enzyme remains stable (100% activity) for 1 h at pH 3.5-7.0. At pH 7.5 and 8.0, the activity drops to about 40% and 20%, respectively. The enzyme is inactive after 1 h at pH 9.0 | Bos grunniens |