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Literature summary for 3.4.23.4 extracted from
- Expression, purification, and characterization of bovine chymosin enzyme using an inducible pTOLT system (2016), Prep. Biochem. Biotechnol., 46, 596-601 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme as a fusion with Tol-A-III protein is expressed in Escherichia coli BL21 (DE3) cells | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | Bos taurus | the enzyme breaks the bond between Phe-105 and Met-106 in the casein and initiates the coagulation of milk | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity column chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stomach | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Bos taurus | ? | - |
? | |
| casein + H2O | the enzyme breaks the bond between Phe-105 and Met-106 in the casein and initiates the coagulation of milk | Bos taurus | ? | - |
? | |
| skimmed milk + H2O | - |
Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35600, active enzyme, calculated from amino acid sequence | Bos taurus |
| ? | x * 40777, proenzyme, calculated from amino acid sequence | Bos taurus |
| ? | x * 48756, Tol-A-III-chymosin fusion protein, calculated from amino acid sequence | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| prochymosin | proenzyme | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
temperature optimum for the Tol-A-III-chymosin fusion protein | Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | the Tol-A-III-chymosin fusion protein shows high activity between 30 and 55°C | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
pH optimum for the Tol-A-III-chymosin fusion protein | Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | the Tol-A-III-chymosin fusion protein shows more than 60% activity between pH 5.0 and 9.0 | Bos taurus |
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Release 2023.1 (January 2023)
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