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Literature summary for 3.4.23.4 extracted from

  • Tyagi, A.; Kumar, A.; Yadav, A.; Chandola Saklani, A.; Grover, S.; Batish, V.
    Functional expression of recombinant goat chymosin in Pichia pastoris bioreactor cultures A commercially viable alternate (2016), LWT-Food Sci. Technol., 69, 217-224 .
No PubMed abstract available
Search for more literature for 3.4.23.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain X-33 Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0178
-
 fluorescein thiocarbamoyl-kappa-casein at pH 5.5 and 37°C Capra hircus

Organism

Organism UniProt Comment Textmining
Capra hircus Q5VK60
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fluorescein thiocarbamoyl-kappa-casein + H2O
-
 Capra hircus ?
-
 ?
skimmed milk + H2O
-
 Capra hircus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Capra hircus

Synonyms

Synonyms Comment Organism
prochymosin proenzyme Capra hircus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 38
-
 Capra hircus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 the enzyme shows more than 90% activity over a temperature range of 30-45°C and about 75% activity at 20°C and 50°C Capra hircus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the recombinant enzyme retains 42% of relative milk clotting activity at 60°C Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 pH optimum for milk-clotting activity Capra hircus

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 the enzyme retains about 65%, 100%, 85% and 69% of milk clotting activity at pH 5.0, 5.5, 6.0 and 6.5, respectively Capra hircus