Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bos taurus |
expressed in Escherichia coli BL21(DE3) cells | Vicugna pacos |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ inhibits milk-clotting activity (about 35% residual activity at 5 mM) | Bos taurus | |
Ca2+ | Ca2+ inhibits milk-clotting activity (about 50% residual activity at 5 mM) | Vicugna pacos |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Vicugna pacos | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
- |
Vicugna pacos |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bovine kappa-casein + H2O | - |
Bos taurus | ? | - |
? | |
bovine kappa-casein + H2O | - |
Vicugna pacos | ? | - |
? | |
milk + H2O | - |
Bos taurus | ? | - |
? | |
milk + H2O | - |
Vicugna pacos | ? | - |
? | |
additional information | in the process of milk coagulation under the action of chymosin, alpha- and beta-caseins are not hydrolyzed | Bos taurus | ? | - |
? | |
additional information | in the process of milk coagulation under the action of chymosin, alpha- and beta-caseins are not hydrolyzed | Vicugna pacos | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rCH | recombinant enzyme | Bos taurus |
rCH | recombinant enzyme | Vicugna pacos |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 60 | the enzyme activity remains stable after 30 min at 35-60°C | Vicugna pacos |
35 | 55 | the enzyme activity remains stable after 30 min at 35-55°C and drops to about 20% after 30 min at 60°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
pH optimum for milk-clotting activity | Bos taurus |
5.5 | - |
pH optimum for milk-clotting activity | Vicugna pacos |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | 100% activity at pH 5.5, about 20% activity at pH 6.0, the enzyme almost completely loses its activity at pH 7.0 | Vicugna pacos |
5.5 | 6 | 100% activity at pH 5.5, about 90% activity at pH 6.0, about 30% activity at pH 6.5, the enzyme almost completely loses its activity at pH 7.0 | Bos taurus |