Cloned (Comment) | Organism |
---|---|
S351T mutant of prochymosin as fusion protein with the Aspergillus niger enzyme glucoamylase in Aspergillus niger var. awamori, insertion of a short peptide linker between the proteins with the sequence TDNST | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
S351T | insertion of a glycosylation site in the linker region between chymosin and glucoamylase, strongly improves enzyme secretion | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | Bos taurus | cleaves a single bond between phenylalanine 105 and methionine 106 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
use of a synthetic gene that encodes a protein identical to bovine prochymosin | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | insertion of a glycosylation site (S351T), glycosylation has significant inhibitory effect but this can be circumvented by deglycosylation with endoglucosidase H | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kappa-casein + H2O | cleaves a single bond between phenylalanine 105 and methionine 106 | Bos taurus | ? | - |
? |