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Literature summary for 3.4.23.39 extracted from

  • Parr-Vasquez, C.L.; Yada, R.Y.
    Functional chimera of porcine pepsin prosegment and Plasmodium falciparum plasmepsin II (2010), Protein Eng. Des. Sel., 23, 19-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information the role of the prosegment in aspartic proteinase structure and function is investigated by generating a chimeric protein consisting of the porcine pepsinogen prosegment fused to the mature region of PMII (pepproPMII): Chimeric protein pepproPMII is capable of both autoactivation and hydrolysis of a synthetic substrate. CD measurements indicate that pepproPMII has reduced thermal stability when compared with zymogen PMII. Prosegment is not critical to obtain a functional enzyme but likely plays a role in protein stabilization Plasmodium falciparum
additional information the role of the prosegment in aspartic proteinase structure and function is investigated by generating a chimeric protein consisting of the prosegment of PMII fused to pepsin (PMIIpropep). Chimeric protein is expressed in Escherichia coli but extremely unstable suggesting protein misfolding Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A inhibits autoactivation Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00017
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH PMII without prosegment, pH 5, temperature not specified in the publication Plasmodium falciparum
0.0007
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication Plasmodium falciparum
0.0012
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH wild-type PMII, pH 5, temperature not specified in the publication Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein is expressed as an inactive zymogen with a large prosegment Plasmodium falciparum

Purification (Commentary)

Purification (Comment) Organism
using nickel affinity chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH + H2O quenched fluorescent peptide substrate Plasmodium falciparum ?
-
?

Synonyms

Synonyms Comment Organism
plasmepsin II
-
Plasmodium falciparum
PMII
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.103
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH PMII without prosegment, pH 5, temperature not specified in the publication Plasmodium falciparum
1.11
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication Plasmodium falciparum
2.1
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH wild-type PMII, pH 5, temperature not specified in the publication Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
optimal pH of wild-type PMII, chimeric PMII and PMII without prosegment Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
3 5 the zpepproPMII chimeric protein (porcine pepsinogen prosegment fused to the mature region of PMII) activates at pH 3-5. Activation at these pH values is consistent with the pH-dependent autoactivation observed for wildtype zymogen PMII Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
620
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH PMII without prosegment, pH 5, temperature not specified in the publication Plasmodium falciparum
1500
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH wild-type PMII, pH 5, temperature not specified in the publication Plasmodium falciparum
1660
-
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication Plasmodium falciparum