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Literature summary for 3.4.23.39 extracted from

  • Moon, R.P.; Tyas, L.; Certa, U.; Rupp, K.; Bur, D.; Jacquet, C.; Matile, H.; Loetscher, H.; Grueninger-Leitch, F.; et al.
    Expression and characterization of plasmepsin I from Plasmodium falciparum (1997), Eur. J. Biochem., 244, 552-560.
    View publication on PubMed
Search for more literature for 3.4.23.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide
-
 Plasmodium falciparum
Ro17-7109
-
 Plasmodium falciparum
Ro40-4388
-
 Plasmodium falciparum
Ro40-5576
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 Lys-Glu-Phe-Val-Phe-Phe(NO2)-Ala-Leu-Lys pH 4.4, 37°C Plasmodium falciparum
0.025
-
 Lys-Glu-Phe-Ile-Phe-Phe(NO2)-Ala-Leu-Lys pH 4.4, 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification plasmepsin II is produced as a precursor. The propart region, about 120 residues, is more than twice as long as those of archetypal zymogens Plasmodium falciparum

Purification (Commentary)

Purification (Comment) Organism
proplasmepsin Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite the proplasmepsin II gene is not transcribed until the later trophozoite stage of parasite growth Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe + H2O
-
 Plasmodium falciparum Leu-Glu-Arg-Ile-Phe + Phe(NO2)-Ser-Phe
-
 ?
Lys-Glu-Phe-Ile-Phe-Phe(NO2)-Ala-Leu-Lys + H2O
-
 Plasmodium falciparum Lys-Glu-Phe-Ile-Phe + Phe(NO2)-Ala-Leu-Lys
-
 ?
Lys-Glu-Phe-Val-Phe-Phe(NO2)-Ala-Leu-Lys + H2O
-
 Plasmodium falciparum Lys-Glu-Phe-Val-Phe + Phe(NO2)-Ala-Leu-Lys
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.05
-
 Lys-Glu-Phe-Val-Phe-Phe(NO2)-Ala-Leu-Lys pH 4.4, 37°C Plasmodium falciparum
15.2
-
 Lys-Glu-Phe-Ile-Phe-Phe(NO2)-Ala-Leu-Lys pH 4.4, 37°C Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 65% of maximal activity, pH 6.0: about 30% of maximal activity, hydrolysis of Leu-Glu-Arg-Ile-Phe-Phe(NO2)-Ser-Phe Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information the Ki-value for Ro17-7109 is above 0.015 mM Plasmodium falciparum
0.00025
-
 Ro40-5576 pH 4.4, 37°C Plasmodium falciparum
0.0007
-
 Ro40-4388 pH 4.4, 37°C Plasmodium falciparum
0.004
-
 N'1-[(E)-(2,3-dihydroxyphenyl)methylidene]-N'3-[(Z)-(2,3-dihydroxyphenyl)methylidene]benzene-1,3-dicarbohydrazide pH 4.4, 37°C Plasmodium falciparum