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Literature summary for 3.4.23.39 extracted from

  • Siripurkpong, P.; Yuvaniyama, J.; Wilairat, P.; Goldberg, D.E.
    Active Site Contribution to Specificity of the Aspartic Proteases Plasmepsins I and II (2002), J. Biol. Chem., 277, 41009-41013.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
engineering a plasmepsin II with the active site surface of plasmepsin I and expression in Escherichia coli Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information engineering a plasmepsin II with the active site surface of plasmepsin I largely maintains the plasmepsin II substrate and inhibitor specificity Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
pepstatin
-
Plasmodium falciparum
SC-50083 IC50: 0.0003467 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000489
-
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Hemoglobin + H2O Plasmodium falciparum enzyme is involved in initial steps of Plasmodium hemoglobin degradation ?
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid + H2O
-
Plasmodium falciparum ?
-
?
Hemoglobin + H2O enzyme is involved in initial steps of Plasmodium hemoglobin degradation Plasmodium falciparum ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.31
-
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid pH 5.0, 37°C Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000037
-
pepstatin pH 5.0, 37°C Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0003467
-
IC50: 0.0003467 mM Plasmodium falciparum SC-50083