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Literature summary for 3.4.23.26 extracted from

  • Lario, L.; Chaud, L.; Almeida, M.; Converti, A.; Duraes Sette, L.; Pessoa, A.
    Production, purification, and characterization of an extracellular acid protease from the marine Antarctic yeast Rhodotorula mucilaginosa L7 (2015), Fungal Biol., 119, 1129-1136 .
No PubMed abstract available
Search for more literature for 3.4.23.26

General Stability

General Stability Organism
the enzyme is stable for 24 h in the presence of high concentrations of NaCl (0.5-3.5 M) Rhodotorula mucilaginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhodotorula mucilaginosa
-
-

Organism

Organism UniProt Comment Textmining
Rhodotorula mucilaginosa
-
-
-
Rhodotorula mucilaginosa L7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CM-Sepharose column chromatography Rhodotorula mucilaginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Rhodotorula mucilaginosa ?
-
 ?
azocasein + H2O
-
 Rhodotorula mucilaginosa L7 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34500, SDS-PAGE Rhodotorula mucilaginosa

Synonyms

Synonyms Comment Organism
extracellular acid protease
-
 Rhodotorula mucilaginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhodotorula mucilaginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 the enzyme retains more than 70 % of activity between 35 and 60°C Rhodotorula mucilaginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the enzyme is highly stable at temperature below 50°C. After 30 min of incubation at 55°C, the residual proteolytic activity is 24% lower than its initial value, and at 60°C the activity loss is 88% Rhodotorula mucilaginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Rhodotorula mucilaginosa

pH Range

pH Minimum pH Maximum Comment Organism
4.3 7.3 the enzyme shows more than 60% activity between pH 4.3 and 7.3 Rhodotorula mucilaginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 the enzyme is stable at pH up to 5.0 and retains on average, 50% of its maximal activity at pH 7.3, but no more than 5% after a 4-h incubation at pH 8.0 Rhodotorula mucilaginosa