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Literature summary for 3.4.23.26 extracted from

  • Murao, S.
    Rhodotorulapepsin (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 139-140.
No PubMed abstract available
Search for more literature for 3.4.23.26

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native enzyme, 3times crystallization Rhodotorula glutinis

Inhibitors

Inhibitors Comment Organism Structure
4-bromo-phenacylbromide
-
 Rhodotorula glutinis
diazoacetyl glycine ethyl ester
-
 Rhodotorula glutinis
Diazoacetyl-DL-norleucine methyl ester i.e. DAN Rhodotorula glutinis
pepstatin Ac 60% inhibition at 0.031 mM, addition to cell culture enhances the cell growth and changes the optimal growth temperature Rhodotorula glutinis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Rhodotorula glutinis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 gel filtration Rhodotorula glutinis
30000
-
 sedimentation equilibrium analysis Rhodotorula glutinis

Organism

Organism UniProt Comment Textmining
Rhodotorula glutinis
-
 isolated from soil
-
Rhodotorula glutinis K-24
-
 isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain K-24 by ammonium sulfate fractionation, acetone precipation, gel filtration to homogeneity Rhodotorula glutinis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rhodotorula glutinis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O high activity Rhodotorula glutinis ?
-
 ?
casein + H2O high activity Rhodotorula glutinis K-24 ?
-
 ?
DRVYIHPFHLLVYS + H2O tetradecapaptide renin substrate, cleavage pattern, overview Rhodotorula glutinis DRVY + IHPFHLL + VYS
-
 ?
DRVYIHPFHLLVYS + H2O tetradecapaptide renin substrate, cleavage pattern, overview Rhodotorula glutinis K-24 DRVY + IHPFHLL + VYS
-
 ?
additional information substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala Rhodotorula glutinis ?
-
 ?
additional information substrate specificity, overview, high activity with increasing chain length and Ala at the N-terminal side of the scissile bond, no activity with Z-Lys-Ala-Ala Rhodotorula glutinis K-24 ?
-
 ?
Z-Lys-Ala-Ala-Ala + H2O
-
 Rhodotorula glutinis Z-Lys + Ala-Ala-Ala
-
 ?
Z-Lys-Ala-Ala-Ala + H2O
-
 Rhodotorula glutinis K-24 Z-Lys + Ala-Ala-Ala
-
 ?
Z-Lys-Leu-Ala-Ala + H2O
-
 Rhodotorula glutinis Z-Lys + Leu-Ala-Ala
-
 ?
Z-Lys-Leu-Ala-Ala + H2O
-
 Rhodotorula glutinis K-24 Z-Lys + Leu-Ala-Ala
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2 2.5
-
 Rhodotorula glutinis

pI Value

Organism Comment pI Value Maximum pI Value
Rhodotorula glutinis isoelectric focusing
-
 4.5