Literature summary for 3.4.23.24 extracted from

Dostal, J.; Dlouha, H.; Malon, P.; Pichova, I.; Hruskova-Heidingsfeldova, O.
The precursor of secreted aspartic proteinase Sapp1p from Candida parapsilosis can be activated both autocatalytically and by a membrane-bound processing proteinase (2005), Biol. Chem., 386, 791-799.

Search for more literature for 3.4.23.24

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Candida parapsilosis

Inhibitors

Inhibitors	Comment	Organism	Structure
isovaleryl-Val-Val-statyl-Ala-statyl-OH	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-Ala-phenylstatyl-O-methyl ester	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-OH	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-O-methyl ester	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-NH2	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-O-methyl ester	-	Candida parapsilosis
tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-OH	-	Candida parapsilosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0092	-	Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu	-	Candida parapsilosis
0.0122	-	Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala	-	Candida parapsilosis
0.0129	-	Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu	-	Candida parapsilosis

Organism

Organism	UniProt	Comment	Textmining
Candida parapsilosis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the Sapp1 precursor can be processed autocatalytically and by a membrane-bound processing proteinase. The pro-segment is indispensable for Sapp1p to attain an appropriate structure	Candida parapsilosis

Purification (Commentary)

Purification (Comment)	Organism
recombinant	Candida parapsilosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala + H2O	-	Candida parapsilosis	Ala-Thr-His-Gln-Val-Tyr + 4-nitrophenylalanine-Val-Arg-Lys-Ala	-	?
Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu + H2O	-	Candida parapsilosis	Lys-Pro-Ala-Glu-Phe + 4-nitrophenylalanine-Ala-Leu	-	?
Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu + H2O	-	Candida parapsilosis	Lys-Pro-Leu-Glu-Met + 4-nitrophenylalanine-Ala-Leu	-	?

Synonyms

Synonyms	Comment	Organism
Sapp1p	-	Candida parapsilosis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.41	-	Ala-Thr-His-Gln-Val-Tyr-4-nitrophenylalanine-Val-Arg-Lys-Ala	-	Candida parapsilosis
24.11	-	Lys-Pro-Leu-Glu-Met-4-nitrophenylalanine-Ala-Leu	-	Candida parapsilosis
31.8	-	Lys-Pro-Ala-Glu-Phe-4-nitrophenylalanine-Ala-Leu	-	Candida parapsilosis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000003	-	isovaleryl-Val-Val-statyl-Ala-statyl-OH	-	Candida parapsilosis
0.0000004	-	tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-O-methyl ester	-	Candida parapsilosis
0.0000009	-	tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-OH	-	Candida parapsilosis
0.00000254	-	tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-NH2	-	Candida parapsilosis
0.0000065	-	tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-O-methyl ester	-	Candida parapsilosis
0.0000066	-	tert-butoxycarbonyl-Val-Val-phenylstatyl-Ala-phenylstatyl-OH	-	Candida parapsilosis
0.005301	-	tert-butoxycarbonyl-Val-Val-(3R,4R)-4-amino-3-hydroxy-5-phenylpentanoyl-Ala-phenylstatyl-O-methyl ester	-	Candida parapsilosis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2026.1 (March 2026)