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Literature summary for 3.4.23.24 extracted from

  • Ruchel, R.
    Properties of a purified proteinase from the yeast Candida albicans (1981), Biochim. Biophys. Acta, 659, 99-113.
    View publication on PubMed
Search for more literature for 3.4.23.24

Activating Compound

Activating Compound Comment Organism Structure
nonionic detergents Triton X-100, Tween-80, Nonidet P-40, slight improvement of activity Candida albicans

General Stability

General Stability Organism
Stable in presence of non-ionic detergents Candida albicans
Stable to freeze-drying Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
1,2-epoxy-3-(4-nitrophenoxy)propane not Candida albicans
additional information methyl 2-diazoacetamidohexanoate Candida albicans
pepstatin
-
 Candida albicans
SDS tolerated at 0.01% w/v, decrease of activity at 0.1% w/v, 1% completely destroys activity within a few min Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 albumin
-
 Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 Candida albicans, nondenaturing PAGE Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Candida albicans CBS-2730
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Candida albicans

Purification (Commentary)

Purification (Comment) Organism
-
 Candida albicans

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Candida albicans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Albumin + H2O
-
 Candida albicans ?
-
 ?
Albumin + H2O
-
 Candida albicans CBS-2730 ?
-
 ?
additional information trypsinogen kinase activity Candida albicans ?
-
 ?
additional information not: horse ferritin Candida albicans ?
-
 ?
additional information clots milk at pH 5.5 Candida albicans ?
-
 ?
additional information trypsinogen kinase activity Candida albicans CBS-2730 ?
-
 ?
additional information not: horse ferritin Candida albicans CBS-2730 ?
-
 ?
additional information clots milk at pH 5.5 Candida albicans CBS-2730 ?
-
 ?
Myeloma proteins of the type A1 and A2 + H2O
-
 Candida albicans ?
-
 ?
Myeloma proteins of the type A1 and A2 + H2O
-
 Candida albicans CBS-2730 ?
-
 ?
Proteinase inhibitors of human serum + H2O
-
 Candida albicans ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, Candida albicans, SDS-PAGE Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 10 min, 85% loss of activity at pH 7.5, 5% loss of activity at pH 6.6 and 7.0 Candida albicans
45
-
 10 min, complete loss of activity Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 3.9 hemoglobin Candida albicans
3.7
-
 bovine serum albumin Candida albicans

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.4
-
 above, alkaline denaturation accompanied by dimerization Candida albicans