Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.23 extracted from

  • Yuqiu, L.; Tanhu, T.; Da, L.; Zhoulin, L.; Yanping, C.; Yuanyuan, J.; Xiangying, L.; Jinghui, W.; Qiyun, L.
    Screening and characterization of a mutant fungal aspartic proteinase from Mucor pusillus (2015), Open Biotechnol. J., 9, 119-126 .
No PubMed abstract available
Search for more literature for 3.4.23.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Rhizomucor pusillus

Protein Variants

Protein Variants Comment Organism
A101T the proteolytic activity of the mutant is similar to the non-mutant enzyme. The milk clotting activity of the mutant is increased compared to the non-mutant enzyme Rhizomucor pusillus
A101T/G186D the proteolytic activity of the mutant is similar to the non-mutant enzyme. The milk clotting activity of the mutant is increased compared to the non-mutant enzyme Rhizomucor pusillus
E13A the proteolytic activity of the mutant decreases remarkably to almost a half of that of non-mutant enzyme Rhizomucor pusillus
E13D the proteolytic activity of the mutant decreases remarkably to almost a half of that of non-mutant enzyme Rhizomucor pusillus
E13P the proteolytic activity of the mutant decreases remarkably to almost a half of that of non-mutant enzyme Rhizomucor pusillus
E13Q the proteolytic activity of the mutant decreases remarkably to almost a half of that of non-mutant enzyme Rhizomucor pusillus
G186D the proteolytic activity of the mutant is similar to the non-mutant enzyme. The milk clotting activity of the mutant is increased compared to the non-mutant enzyme Rhizomucor pusillus
G186D/E13D the mutant shows a significant milk-clotting activity. The mutant rennet can decrease hydrolysis of protein during ripening of cheese. The mutant has a reduction in thermostability along with a sharp decrease in proteolytic activity Rhizomucor pusillus
G186D/E13Q the mutant has a reduction in thermostability along with a sharp decrease in proteolytic activity Rhizomucor pusillus
G186D/E13Q the mutant shows a significant milk-clotting activity. The mutant rennet can decrease hydrolysis of protein during ripening of cheese. The mutant has a reduction in thermostability along with a sharp decrease in proteolytic activity Rhizomucor pusillus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 1 mM Rhizomucor pusillus
Aprotinin 72.6% residual activity at 1 mM Rhizomucor pusillus
EDTA 71.1% residual activity at 50 mM Rhizomucor pusillus
Fe2+ 87.7% residual activity at 5 mM Rhizomucor pusillus
Fe3+ 85.8% residual activity at 5 mM Rhizomucor pusillus
leupeptin 96.5% residual activity at 0.2 mM Rhizomucor pusillus
additional information not inhibited by Cu2+ and Na+ Rhizomucor pusillus
Ni2+ 47.8% residual activity at 5 mM Rhizomucor pusillus
pepstatin A 24.5% residual activity at 0.05 mM Rhizomucor pusillus
phenylmethylsulfonyl fluoride 93.1% residual activity at 1 mM Rhizomucor pusillus
Zn2+ 85.5% residual activity at 5 mM Rhizomucor pusillus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 218.8% activity at 5 mM Rhizomucor pusillus
K+ 117.3% activity at 5 mM Rhizomucor pusillus
Mg2+ 106.8% activity at 5 mM Rhizomucor pusillus
Mn2+ 120.7% activity at 5 mM Rhizomucor pusillus

Organism

Organism UniProt Comment Textmining
Rhizomucor pusillus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-52 column chromatography and Sephadex G-100 gel filtration Rhizomucor pusillus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Rhizomucor pusillus ?
-
 ?
skimmed milk + H2O
-
 Rhizomucor pusillus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Rhizomucor pusillus

Synonyms

Synonyms Comment Organism
Aspartic proteinase
-
 Rhizomucor pusillus
MPR
-
 Rhizomucor pusillus
Mucor rennin
-
 Rhizomucor pusillus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 purified mutant G186D/E13D and non-mutant enzymes act optimally at 60°C and later, they start losing their activity rapidly leading to complete inactivation at 75°C Rhizomucor pusillus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 the wild type enzyme retains 88.6% activity after 60 min at 55°C. Mutant enzyme G186D/E13D loses 30% of its activity after 50 min at 55°C Rhizomucor pusillus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Rhizomucor pusillus

pH Range

pH Minimum pH Maximum Comment Organism
5 7 about 85% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.0, about 65% activity at pH 6.5, about 40% activity at pH 7.0 Rhizomucor pusillus