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Literature summary for 3.4.23.20 extracted from

  • Smirnova, I.; Sereda, A.; Kostyleva, E.; Tsurikova, N.; Bushina, E.; Rozhkova, A.; Sinitsyn, A.
    A new enzyme preparation with high penicillopepsin activity based on the producer strain Penicillium canescens (2015), Appl. Biochem. Microbiol., 51, 660-666 .
    View publication on PubMed
Search for more literature for 3.4.23.20

Organism

Organism UniProt Comment Textmining
Penicillium canescens
-
-
-
Penicillium canescens RN3-11-7 niaD(-)
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Penicillium canescens ?
-
 ?
Hemoglobin + H2O
-
 Penicillium canescens RN3-11-7 niaD(-) ?
-
 ?
wheat flour + H2O
-
 Penicillium canescens ?
-
 ?
wheat flour + H2O
-
 Penicillium canescens RN3-11-7 niaD(-) ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Penicillium canescens

Synonyms

Synonyms Comment Organism
PEP
-
 Penicillium canescens
PepA
-
 Penicillium canescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penicillium canescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 about 50% activity at 30°C, about 80% activity at 40°C, 100% activity at 50°C, about 35% activity at 60°C Penicillium canescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme remains stable after 80 min incubation at 30-50°C (pH 5.0). The activity drops to less than 20% after 80 min at 60°C. The enzyme shows about 75% activity after 2 h at 50°C Penicillium canescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Penicillium canescens

pH Range

pH Minimum pH Maximum Comment Organism
3 6 about 45% activity at pH 3.0, 100% activity at pH 4.0, about 90% activity at pH 5.0, about 30% activity at pH 6.0 Penicillium canescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 the enzyme maintains more than 70% activity after incubation for 24 h at pH 3.0-7.0 25°C Penicillium canescens