Cloned (Comment) | Organism |
---|---|
gene pepA, nucleotide sequence determined, inserted into expression vector pGPT-pyrG1, expressed in an aspartic proteinase-free strain of Aspergillus niger var. awamori | Penicillium janthinellum |
Protein Variants | Comment | Organism |
---|---|---|
T219A | constructed by cassette insertion | Penicillium janthinellum |
T219G | constructed by cassette insertion | Penicillium janthinellum |
T219S | constructed by cassette insertion | Penicillium janthinellum |
T219V | constructed by cassette insertion | Penicillium janthinellum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin | - |
Penicillium janthinellum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.3 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.32 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.35 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.37 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, and mutant T219S | Penicillium janthinellum | |
0.39 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291S | Penicillium janthinellum | |
0.4 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.4 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.4 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.41 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.42 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.46 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.46 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.46 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.48 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.48 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.48 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.5 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.5 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.5 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.5 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.51 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.54 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.55 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.55 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291G | Penicillium janthinellum | |
0.57 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.59 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.59 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291V | Penicillium janthinellum | |
0.59 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.6 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.6 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.6 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219G and T219S | Penicillium janthinellum | |
0.6 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.6 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.65 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.68 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291A | Penicillium janthinellum | |
0.71 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V and T219A | Penicillium janthinellum | |
0.74 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.75 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.77 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.8 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.8 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.83 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.83 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33700 | 34000 | mass spectrometry | Penicillium janthinellum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium janthinellum | - |
- |
- |
Penicillium janthinellum NRRL 905 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | PencillopepsinJT2, glycosylated form of the active enzyme | Penicillium janthinellum |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Penicillium janthinellum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Ala-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum NRRL 905 | ? | - |
? | |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Lys-(NO2)Phe-Ala-amide + H2O | - |
Penicillium janthinellum NRRL 905 | ? | - |
? | |
Ac-Ala-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Ala-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum NRRL 905 | ? | - |
? | |
Ac-Ala-Lys-Ala-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Lys-(NO2)Phe- Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Lys-(NO2)Phe- Ala-amide + H2O | - |
Penicillium janthinellum NRRL 905 | ? | - |
? | |
Ac-Lys-(NO2)Phe-Ala-Ala-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum | ? | - |
? | |
Ac-Lys-(NO2)Phe-amide + H2O | - |
Penicillium janthinellum NRRL 905 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pencillopepsin-JT1 | native enzyme | Penicillium janthinellum |
pencillopepsin-JT2 | recombinant enzyme | Penicillium janthinellum |
Penicillium janthinellum aspartic proteinase | - |
Penicillium janthinellum |
Peptidase A | - |
Penicillium janthinellum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.0019 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, and mutant T219V | Penicillium janthinellum | |
0.002 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 and mutant T219A | Penicillium janthinellum | |
0.003 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.003 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.003 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.003 | - |
Ac-Ala-Lys-Ala-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.003 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
0.004 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.005 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.005 | - |
Ac-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.0054 | - |
Ac-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.042 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.046 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.05 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.07 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.09 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.09 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.11 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.12 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.12 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.13 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.14 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.15 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.17 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.23 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.24 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.29 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
0.3 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.3 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V | Penicillium janthinellum | |
0.3 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
0.33 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
0.35 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.35 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291V | Penicillium janthinellum | |
0.36 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291A | Penicillium janthinellum | |
0.36 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
0.4 | - |
Ac-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
0.43 | - |
Ac-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219A | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219V and T219G | Penicillium janthinellum | |
0.45 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291G | Penicillium janthinellum | |
0.5 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219G | Penicillium janthinellum | |
0.6 | - |
Ac-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
3.8 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
14.9 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T219S | Penicillium janthinellum | |
15 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
16.1 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, mutant T291S | Penicillium janthinellum | |
34 | - |
Ac-Ala-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum | |
40 | - |
Ac-Ala-Ala-Lys-(NO2)Phe-Ala-Ala-amide | pH 5.0, 25°C, wild-type pencillopepsin JT-2 | Penicillium janthinellum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000001 | - |
pepstatin | pH 5.0, 37°C, wild-type Pencillopepsin JT-2 | Penicillium janthinellum | |
0.0000004 | - |
pepstatin | pH 5.0, 37°C, mutant T219S | Penicillium janthinellum | |
0.00055 | - |
pepstatin | pH 5.0, 37°C, mutant T219A | Penicillium janthinellum | |
0.0016 | - |
pepstatin | pH 5.0, 37°C, mutant T219V | Penicillium janthinellum | |
0.0018 | - |
pepstatin | pH 5.0, 37°C, mutant T219G | Penicillium janthinellum |