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Literature summary for 3.4.23.19 extracted from
- Specific inhibition and stabilization of aspergilloglutamic peptidase by the propeptide. Identification of critical sequences and residues in the propeptide (2005), J. Biol. Chem., 280, 999-1006.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| synthetic propeptides | wild-type sequence Ala1-Asn41 and diverse truncated or mutated propeptides, overview, Ala1-Asn41 shows specific competitive inhibition, while mutants, especially R19A and H20A, show weak inhibitory effects, detailed overview | Aspergillus niger var. macrosporus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, thermodynamics, overview | Aspergillus niger var. macrosporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger var. macrosporus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is synthesized as preproform and performs autoprocessing to the mature enzyme removing the 41-amino acid propeptide and an 11-amino acid intervening peptide | Aspergillus niger var. macrosporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| angiotensin II + H2O | - |
Aspergillus niger var. macrosporus | DRVYIH + Pro-Phe | - |
? |  |
| substance P + H2O | - |
Aspergillus niger var. macrosporus | RPKPQQFF + Gly-Leu-Met | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGP | - |
Aspergillus niger var. macrosporus |
| aspergilloglutamic peptidase | - |
Aspergillus niger var. macrosporus |
| More | the enzyme belongs to the G1 peptidase family | Aspergillus niger var. macrosporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus niger var. macrosporus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal stability overview, kinetics, thermodynamics, thermal unfolding is reversible at pH 5.0-6.0, overview, the wild-type propeptide stabilizes the enzyme, effects of truncated or mutated propeptides, overview | Aspergillus niger var. macrosporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
assay at | Aspergillus niger var. macrosporus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH profile | Aspergillus niger var. macrosporus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics of truncated or mutated propeptides, detailed overview | Aspergillus niger var. macrosporus | |
| 0.000027 | - |
synthetic propeptide Ala1-Asn24 | pH 4.0, 30°C | Aspergillus niger var. macrosporus |
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Release 2023.1 (January 2023)
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