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Literature summary for 3.4.23.19 extracted from
- Identification of a glutamine residue essential for catalytic activity of aspergilloglutamic peptidase by site-directed mutagenesis (2004), FEBS Lett., 569, 161-164.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes | Aspergillus niger var. macrosporus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D123N | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| E219A | site-directed mutagenesis, proform mutant, inactive mutant | Aspergillus niger var. macrosporus |
| E219Q | site-directed mutagenesis, proform mutant, inactive mutant | Aspergillus niger var. macrosporus |
| N213A | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| N65A | site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| Q133A | site-directed mutagenesis, proform mutant, inactive mutant | Aspergillus niger var. macrosporus |
| Q133E | site-directed mutagenesis, proform mutant, inactive mutant | Aspergillus niger var. macrosporus |
| Q133E/E219Q | site-directed mutagenesis, proform mutant, inactive mutant | Aspergillus niger var. macrosporus |
| S162A | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| S167A | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| T126A | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| T134A | site-directed mutagenesis, proform mutant, the mutant shows similar activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| T192A | site-directed mutagenesis, proform mutant, the mutant shows increased activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| T268A | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| Y151F | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| Y154F | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
| Y291F | site-directed mutagenesis, proform mutant, the mutant shows reduced activity compared to the wild-type enzyme | Aspergillus niger var. macrosporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger var. macrosporus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is synthesized as preproform and performs autoprocessing to the mature enzyme | Aspergillus niger var. macrosporus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes including denaturation and refolding | Aspergillus niger var. macrosporus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| preferential cleavage in B chain of insulin: Asn3-/-Gln, Gly13-/-Ala, Tyr26-/-Thr | catalytic residues are Glu219 and Glu133, numbering from the preproform, Glu219 and Glu133 form a catalytic dyad | Aspergillus niger var. macrosporus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes, denaturation by 8 M urea, refolding after dialysis in 50 mM sodium acetate, pH 5.3, 10 mM 2-mercaptoethanol, at 4°C overnight | Aspergillus niger var. macrosporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activities of wild-type and mutant enzymes, overview | Aspergillus niger var. macrosporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Aspergillus niger var. macrosporus | ? | - |
? |  |
| Hemoglobin + H2O | - |
Aspergillus niger var. macrosporus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AGP | - |
Aspergillus niger var. macrosporus |
| aspergilloglutamic peptidase | - |
Aspergillus niger var. macrosporus |
| More | the enzyme belongs to the G1 peptidase family | Aspergillus niger var. macrosporus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
substrate hemoglobin | Aspergillus niger var. macrosporus |
| 2 | - |
substrate casein | Aspergillus niger var. macrosporus |
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