Crystallization (Comment) | Organism |
---|---|
wild-type and mutant A71V/V82T/I84V enzymes in complex with inhibitors SE, and SQ, hanging drop vapour diffusion method, 25°C, the reservoir solution contains 0.1 M sodium citrate, pH 4.4, 0.5 M sodium chloride, and 10% v/v glycerol, X-ray diffraction structure determination and analysis at 1.93-2.38 A resolution | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
A71V/V82T/I84V | site-directed mutagenesis, the mutant enzyme shows altered interactions with inhibitors compared to the wild-type enzyme | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tert-butoxycarbonyl-Phe-psi[CH2CH2NH]-Phe-Glu-Phe-NH2 | i.e. tert-butoxycarbonyl-Phe-psi[CH2CH2NH]-Phe-Glu-Phe-NH2, an ethyleneamine inhibitor, interactions with the enzyme, overview | Human immunodeficiency virus 1 | |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Gln-Phe-NH2 | i.e. tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Gln-Phe-NH2, a pseudotetrapeptide inhibitor with an acyclic S-hydroxyethylamine isostere, interactions with the enzyme and binding structure, overview | Human immunodeficiency virus 1 | |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Glu-Phe-NH2 | i.e. tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Glu-Phe-NH2, a pseudotetrapeptide inhibitor with an acyclic S-hydroxyethylamine isostere, interactions with the enzyme and binding structure, overview | Human immunodeficiency virus 1 | |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2]-Phe-Gln-Phe-NH2 | i.e. tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2]-Phe-Gln-Phe-NH2, a hydroxyethylene inhibitor, interactions with the enzyme, overview | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
i.e. HIV-1 | - |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 protease | - |
Human immunodeficiency virus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000002 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2]-Phe-Gln-Phe-NH2 | wild-type enzyme | Human immunodeficiency virus 1 | |
0.00000015 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Glu-Phe-NH2 | wild-type enzyme | Human immunodeficiency virus 1 | |
0.00000026 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2]-Phe-Gln-Phe-NH2 | mutant A71V/V82T/I84V enzyme | Human immunodeficiency virus 1 | |
0.0000015 | - |
tert-butoxycarbonyl-Phe-psi[CH2CH2NH]-Phe-Glu-Phe-NH2 | wild-type enzyme | Human immunodeficiency virus 1 | |
0.0000041 | - |
tert-butoxycarbonyl-Phe-psi[CH2CH2NH]-Phe-Glu-Phe-NH2 | mutant A71V/V82T/I84V enzyme | Human immunodeficiency virus 1 | |
0.000033 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Gln-Phe-NH2 | wild-type enzyme | Human immunodeficiency virus 1 | |
0.000086 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Glu-Phe-NH2 | mutant A71V/V82T/I84V enzyme | Human immunodeficiency virus 1 | |
0.001 | - |
tert-butoxycarbonyl-Phe-psi[S-CH(OH)CH2NH]-Phe-Gln-Phe-NH2 | mutant A71V/V82T/I84V enzyme | Human immunodeficiency virus 1 |