Cloned (Comment) | Organism |
---|---|
expression of the enzyme in HEK-293T cells, coexpression with tRNALys2 or tRNALys3, and with viral GagPol protein | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lysyl-tRNA synthetase | - |
Human immunodeficiency virus 1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gag protein + H2O | Human immunodeficiency virus 1 | proteolytic cleavage in the host cell cytoplasm | ? | - |
? | |
Gag-Pol polyprotein + H2O | Human immunodeficiency virus 1 | proteolytic cleavage in the host cell cytoplasm | ? | - |
? | |
additional information | Human immunodeficiency virus 1 | the enzyme is involved in regulation of the incorporation of reverse transcriptase in the viral early assembly complex comprising reverse transcriptase, genomic RNA, Gag, Gag-Pol, tRNALys, and lysyl tRNA synthetase, HIV-1 protease activity is negatively regulated by the lysyl tRNA synthetase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
i.e. HIV-1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme activity measurement by bioluminescence resonance energy transfer analysis | Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gag protein + H2O | proteolytic cleavage in the host cell cytoplasm | Human immunodeficiency virus 1 | ? | - |
? | |
Gag protein + H2O | site-specific proteolytic cleavage of the protein precursor | Human immunodeficiency virus 1 | ? | - |
? | |
Gag-Pol polyprotein + H2O | proteolytic cleavage in the host cell cytoplasm | Human immunodeficiency virus 1 | ? | - |
? | |
Gag-Pol polyprotein + H2O | site-specific proteolytic cleavage of the protein precursor | Human immunodeficiency virus 1 | ? | - |
? | |
additional information | the enzyme is involved in regulation of the incorporation of reverse transcriptase in the viral early assembly complex comprising reverse transcriptase, genomic RNA, Gag, Gag-Pol, tRNALys, and lysyl tRNA synthetase, HIV-1 protease activity is negatively regulated by the lysyl tRNA synthetase | Human immunodeficiency virus 1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 protease | - |
Human immunodeficiency virus 1 |