Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method at 20°C, A28S mutant enzyme in complex with the peptide inhibitor U-89360E | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
A28S | the ratio of turnover number to Km-value for the substrate TATIMMQRGN is 1527fold lower than that for the wild-type enzyme. The ratio of turnover number to Km-value for the substrate RQGTVSFNFPQITL is 1488fold lower than that for the wild-type enzyme | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
U-89360E | - |
Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
TATIMMQRGN | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
0.34 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
0.54 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Human immunodeficiency virus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RQGTVSFNFPQITL + H2O | - |
Human immunodeficiency virus 1 | RQGTVSFNF + PQITL | - |
? | |
TATIMMQRGN + H2O | - |
Human immunodeficiency virus 1 | TATIM + MQRGN | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
TATIMMQRGN | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
0.026 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
58.8 | - |
TATIMMQRGN | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
62.7 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |