Protein Variants | Comment | Organism |
---|---|---|
A28S | with VSQNYPIVQ the turnover-number of the mutant enzyme is 37% of that of the wild-type enzyme | Human immunodeficiency virus 1 |
G48H | with VSQNYPIVQ the turnover-number of the mutant enzyme is 60% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 2.2fold higher than the Km-value of the wild-type enzyme | Human immunodeficiency virus 1 |
G48V | the kcat/Km ratio for the substrate KARVLAEAMS is 55% of that for the autoproteolysis resistant variant Q7K/L33I/L63I/C67A/C95A | Human immunodeficiency virus 1 |
G48V | mutant enzyme with 9.3% of the activity of the wild-type enzyme with VSQNYPIVQ as substrate | Human immunodeficiency virus 1 |
I84V | with VSQNYPIVQ the turnover-number of the mutant enzyme is 19% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 7.2fold higher than the Km-value of the wild-type enzyme | Human immunodeficiency virus 1 |
L90M | with VSQNYPIVQ the turnover-number of the mutant enzyme is 6% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 76% of the Km-value of the wild-type enzyme | Human immunodeficiency virus 1 |
V82A | with VSQNYPIVQ the turnover-number of the mutant enzyme is 40% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 2.8fold higher than the Km-value of the wild-type enzyme | Human immunodeficiency virus 1 |
V82E | with VSQNYPIVQ the turnover-number of the mutant enzyme is 60% of that of the wild-type enzyme, with VSQNYPIVQ the KM-value of the mutant enzyme is 6.9fold higher than the Km-value of the wild-type enzyme | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
0.54 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
1.15 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme L90M | Human immunodeficiency virus 1 | |
1.5 | - |
VSQNYPIVQ | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
3.3 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme G48H | Human immunodeficiency virus 1 | |
3.8 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme G48V/L90M | Human immunodeficiency virus 1 | |
4.2 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme V82A | Human immunodeficiency virus 1 | |
6.4 | - |
KLRPGNFLQSRP | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KGRPGNFLQSRP + H2O | - |
Human immunodeficiency virus 1 | KGRPGNF + LQSRP | - |
? | |
KLRPGNFLQSRP + H2O | - |
Human immunodeficiency virus 1 | KLRPGNF + LQSRP | - |
? | |
RQGTVSFNFPQITL + H2O | - |
Human immunodeficiency virus 1 | RQGTVSFNF + PQITL | - |
? | |
VSQNYPIVQ + H2O | - |
Human immunodeficiency virus 1 | VSQNY + PIVQ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme I84V | Human immunodeficiency virus 1 | |
0.172 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme V82E | Human immunodeficiency virus 1 | |
2.2 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme G48V/L90M | Human immunodeficiency virus 1 | |
3.14 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme L90M | Human immunodeficiency virus 1 | |
9.7 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme I82V | Human immunodeficiency virus 1 | |
12.7 | - |
KLRPGNFLQSRP | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
19.2 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme A28S | Human immunodeficiency virus 1 | |
20.7 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme V82A | Human immunodeficiency virus 1 | |
30.9 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme V82E | Human immunodeficiency virus 1 | |
31.3 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme G48V | Human immunodeficiency virus 1 | |
51.8 | - |
VSQNYPIVQ | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
62.7 | - |
RQGTVSFNFPQITL | pH 5.0, 37°C, wild-type enzyme | Human immunodeficiency virus 1 | |
93.5 | - |
VSQNYPIVQ | pH 5.0, 37°C, mutant enzyme G48H | Human immunodeficiency virus 1 |