Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol | Nepenthes distillatoria |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | isoforms nethepsin I, IIa and IIb are completely inactivated in the presence of 40-70 mM dithiothreitol | Nepenthes alata |
guanidine-HCl | completely inactivated in the presence of 3 M, the midpoints of denaturation are 4.3 M, 0.4 M and 1.2 M guanidine-HCl for isoform nethepsin I, IIa and IIb, respectively | Nepenthes alata |
guanidine-HCl | completely inactivated in the presence of 3 M, the midpoints of denaturation are 4.3 M, 0.4 M and 1.2 M guanidine-HCl for isoform nethepsin I, IIa and IIb, respectively | Nepenthes distillatoria |
urea | activity is completely lost in the presence of 5 M urea, the midpoints of denaturation are 1.7 M, 2.4 M and 1.6 M urea for isoform nethepsin I, IIa and IIb, respectively | Nepenthes alata |
urea | activity is completely lost in the presence of 5 M urea, the midpoints of denaturation are 1.7 M, 2.4 M and 1.6 M urea for isoform nethepsin I, IIa and IIb, respectively | Nepenthes distillatoria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepenthes alata | - |
- |
- |
Nepenthes distillatoria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, pepstatin column chromatography, MonoQ column chromatography, and Sephacryl S-200 gel filtration | Nepenthes alata |
DEAE-cellulose column chromatography, pepstatin column chromatography, MonoQ column chromatography, and Sephacryl S-200 gel filtration | Nepenthes distillatoria |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pitcher secretion | - |
Nepenthes alata | - |
pitcher secretion | - |
Nepenthes distillatoria | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Nepenthes alata | ? | - |
? | |
Hemoglobin + H2O | - |
Nepenthes distillatoria | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nepenthesin I | isoform | Nepenthes alata |
nepenthesin I | isoform | Nepenthes distillatoria |
nepenthesin IIa | isoform | Nepenthes alata |
nepenthesin IIa | isoform | Nepenthes distillatoria |
nepenthesin IIb | isoform | Nepenthes alata |
nepenthesin IIb | isoform | Nepenthes distillatoria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
53 | - |
stable up to 53°C during 1 h of incubation | Nepenthes alata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.9 | - |
at pH 2.9 at 37°C, activity decreased very slowly on longer incubation, about 60% and 30% of the original activity are retained after 30 days and 65 days, respectively | Nepenthes alata |
3 | 10 | when nepenthesin I is incubated at pH 3.0 at 60°C for 30 days, about 30% of the original activity remains. When incubated at pH 3.0-10.0 at 37°C for 30 days, the enzyme retains 80% or more of activity | Nepenthes distillatoria |