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Literature summary for 3.4.23.12 extracted from
- Digestive enzymes in insectivorous plants. III. Acid proteases in the genus Nepenthes and Drosera peltata (1972), J. Biochem., 72, 73-81.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptides + H2O | Drosera peltata | preferential cleavage: -Asp, Asp-, Lys-, Ala- | ? | - |
? |  |
| Peptides + H2O | Nepenthes sp. | preferential cleavage: -Asp, Asp-, Lys-, Ala- | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosera peltata | - |
- |
- |
| Nepenthes sp. | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Similar to pepsin, but also cleaves on either side of Asp and at Lys-/-Arg | splits peptide bonds at carboxyl and amino sides of aspartic acid residue, at carboxyl side of alanine and probably carboxyl side of lysine | Drosera peltata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| LFNQDVDAAVR + H2O | - |
Nepenthes sp. | LFNQDVD + AAVR | - |
? | |
| LQQKRWDEAA + H2O | - |
Nepenthes sp. | LQQK + L-Arg-L-Trp + DEAA | - |
? | |
| NQDVDAAVRGIK + H2O | - |
Nepenthes sp. | NQDV + L-Asp + AAVRGIK | - |
? | |
| Peptides + H2O | preferential cleavage: -Asp, Asp-, Lys-, Ala- | Drosera peltata | ? | - |
? | |
| Peptides + H2O | preferential cleavage: -Asp, Asp-, Lys-, Ala- | Nepenthes sp. | ? | - |
? | |
| TGTWDAYK + H2O | - |
Nepenthes sp. | TGTWDA + YK | - |
? | |
| Thr-Ile-Asp-Glu + H2O | - |
Nepenthes sp. | Thr-Ile-Asp + Glu | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Drosera peltata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 3.0-9.0 | Drosera peltata |
| 60 | - |
highest stability at pH 5 | Drosera peltata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Drosera peltata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.7 | 3.2 | - |
Drosera peltata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 40°C, highest stability at pH 3.0-9.0 | Drosera peltata |
| 5 | - |
60°C, highest stability at pH 5 | Drosera peltata |
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Release 2023.1 (January 2023)
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