Cloned (Comment) | Organism |
---|---|
recombinant expression in Pichia pastoris | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nicotinamide | complete inhibition at 25 mM | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Trypanosoma brucei | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, recombinant zymogen, SDS-PAGE | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q6R7Z5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | Z-Arg-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant zymogen, SDS-PAGE | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
CatB | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Trypanosoma brucei |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of the lysosomal cathepsin B-like protease blocks endocytosis causing cell death. Nicotinamide causes cytokinesis defects and cell cycle arrest at G2 phase | Trypanosoma brucei |
physiological function | cathepsin B-like is a lysosomal protease responsible for degradation of host-derived growth factors, such as transferrin, in the bloodstream form of Trypanosoma brucei | Trypanosoma brucei |