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Literature summary for 3.4.22.B6 extracted from

  • Mendoza-Palomares, C.; Biteau, N.; Giroud, C.; Coustou, V.; Coetzer, T.; Authie, E.; Boulange, A.; Baltz, T.
    Molecular and biochemical characterization of a cathepsin B-like protease family unique to Trypanosoma congolense (2008), Eukaryot. Cell, 7, 684-697.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B6

Activating Compound

Activating Compound Comment Organism Structure
DTT no activity in buffer lacking DTT Trypanosoma congolense

Application

Application Comment Organism
medicine TcoCBc1 elicits an important immune response in experimentally infected cattle. The protein is a potential therapeutic target and is a plausible antigen for Trypanosoma congolense diagnosis Trypanosoma congolense

Cloned(Commentary)

Cloned (Comment) Organism
six-His-tagged protein is expressed in Pichia pastoris strain X-33 Trypanosoma congolense

Inhibitors

Inhibitors Comment Organism Structure
acetyl-Leu-Val-lysinal 350 nM, 37°C, pH 4.5, 1 h, 15% inhibition Trypanosoma congolense
CA074 350 nM, 37°C, pH 4.5, 1 h, complete inhibition Trypanosoma congolense
CBZ-Phe-Gly-NHO-Bz-pMe 350 nM, 37°X, pH 4.5, 1 h, 87% inhibition Trypanosoma congolense
cystatin 350 nM, 37°C, pH 4.5, 1 h, 23% inhibition Trypanosoma congolense
E-64 350 nM, 37°C, pH 4.5, 1 h, complete inhibition Trypanosoma congolense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1234
-
 Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
0.1733
-
 N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Trypanosoma congolense 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 39000 Da and a minor protein of 40000 Da. These bands correspond to glycosylated forms of a 35000 Da protein, the expected size of the protease Trypanosoma congolense
additional information
-
 SDS-PAGE analysis of the eluted recombinant protein reveals a prominent band of 40000 Da and a minor protein of 50000 Da. These bands correspond to glycosylated forms of a 36000 Da protein, the expected size of the protease Trypanosoma congolense

Organism

Organism UniProt Comment Textmining
Trypanosoma congolense
-
-
-
Trypanosoma congolense B2C323
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification TcoCBc1 is able to autocatalyze its maturation 21 residues upstream of the predicted start of the catalytic domain Trypanosoma congolense
proteolytic modification TcoCBc6 is able to autocatalyze its maturation 31 residues, upstream of the predicted start of the catalytic domain Trypanosoma congolense

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma congolense

Source Tissue

Source Tissue Comment Organism Textmining
bloodstream form
-
 Trypanosoma congolense
-
bloodstream form expressed mainly in bloodstream form Trypanosoma congolense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-Phe-Arg-Ala-(2,4-dinitrophenyl)-epsilon-NH2-lysine + H2O
-
 Trypanosoma congolense ?
-
 ?
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense CBZ-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information no hydrolysis of benzoyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin and benzoyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin Trypanosoma congolense ?
-
 ?
Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Trypanosoma congolense Nalpha-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
CBc1
-
 Trypanosoma congolense
CBc6
-
 Trypanosoma congolense
TcoCBc1
-
 Trypanosoma congolense
TcoCBc6
-
 Trypanosoma congolense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.09
-
 Nalpha-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
3 6 CBZ-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense
3.49
-
 N2-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin
-
 Trypanosoma congolense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Trypanosoma congolense

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5 pH 4.0: about 30% of maximal activity, pH 6.5: about 75% of maximal activity Trypanosoma congolense