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Literature summary for 3.4.22.B28 extracted from

  • Hata, S.; Doi, N.; Kitamura, F.; Sorimachi, H.
    Stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains (2007), J. Biol. Chem., 282, 27847-27856.
    View publication on PubMed
Search for more literature for 3.4.22.B28

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+-dependent activity, with half-maximal activity at about 0.3 mM Ca2+ Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
stomach nCL-2/calpain 8 is predominantly expressed in the stomach, where it appears to be involved in membrane trafficking in the gastric surface mucus cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calpain 8 + H2O one of the autolysis sites is between Ala5 and Ala6 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
monomer stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains. nCL-2 exists in both monomeric and homo-oligomeric forms, but not as a heterodimer with 30K or 30K-2, and that the oligomerization occurs through domains other than the 5EF-hand domain IV, most probably through domain III Mus musculus
oligomer stomach-specific calpain, nCL-2/calpain 8, is active without calpain regulatory subunit and oligomerizes through C2-like domains. nCL-2 exists in both monomeric and homo-oligomeric forms, but not as a heterodimer with 30K or 30K-2, and that the oligomerization occurs through domains other than the 5EF-hand domain IV, most probably through domain III Mus musculus

Synonyms

Synonyms Comment Organism
nCL-2/calpain 8
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 optimal temperature for nCL-2 activity is 20°C. The time courses of nCL-2 activity at various temperatures shows that the initial rates are maximal at 30-37°C, whereas maximal activity is achieved at 15°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Mus musculus