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Literature summary for 3.4.22.8 extracted from

  • Fortier, G.; MacKenzie, S.L.
    Peptide bond synthesis by clostridiopeptidase B (1986), Biotechnol. Lett., 8, 777-782.
No PubMed abstract available
Search for more literature for 3.4.22.8

Application

Application Comment Organism
synthesis the enzyme can be used for the high yielding synthesis of a variety of peptide bond using L-Arg and, to a lesser degree, L-Lys as substrates with L-amino acid amides or D-amino acid amides Hathewaya histolytica

Organism

Organism UniProt Comment Textmining
Hathewaya histolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbobenzoxy-Arg methyl ester + L-Pro-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-Pro-NH2 + methanol
-
 ?
carbobenzoxy-L-Arg methyl ester + D-Phe-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-D-Phe-NH2 + methanol
-
 ?
carbobenzoxy-L-Arg methyl ester + D-Trp-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-D-Trp-NH2 + methanol
-
 ?
carbobenzoxy-L-Arg methyl ester + Gly-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-Gly-NH2 + methanol
-
 ?
carbobenzoxy-L-Arg methyl ester + L-Phe-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-Phe-NH2 + methanol
-
 ?
carbobenzoxy-L-Arg methyl ester + L-Trp-NH2 no activity with carbobenzoxy-D-Arg methyl ester Hathewaya histolytica carbobenzoxy-Arg-Trp-NH2 + methanol
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 synthesis of carbobenzoxy-Arg-Gly-NH2 Hathewaya histolytica
7.5
-
 synthesis of carbobenzoxy-Arg-Pro-NH2 Hathewaya histolytica

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: about 35% of maximal activity, pH 8.5: about 70% of maximal activity, synthesis of carbobenzoxy-Arg-Gly-NH2 Hathewaya histolytica
7 8.5 pH 7.0: about 40% of maximal activity, pH 8.5: about 50% of maximal activity, synthesis of carbobenzoxy-Arg-Pro-NH2 Hathewaya histolytica