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Literature summary for 3.4.22.70 extracted from

  • Dasgupta, S.; Samantaray, S.; Sahal, D.; Roy, R.P.
    Isopeptide ligation catalyzed by quintessential sortase A: mechanistic cues from cyclic and branched oligomers of indolicidin (2011), J. Biol. Chem., 286, 23996-24006.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18
-
 agmatine at 37°C, pH not specified in the publication, transpeptidation reaction Staphylococcus aureus
1.59
-
 YALPETGK at 37°C, pH not specified in the publication Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + YALPETGK transpeptidase reaction. The initially formed acyl-enzyme intermediate undergoes a hydrolysis followed by intra-molecular transpeptidation Staphylococcus aureus (NH2)2-CN-(CH2)4-NH-(CO)-TEPLAY + ?
-
 ?
additional information sortase A also mediates transpeptidation reaction of indolicidin-derived peptides Staphylococcus aureus ?
-
 ?
YALPETGK + H2O
-
 Staphylococcus aureus YALPET + Gly-Lys
-
 ?

Synonyms

Synonyms Comment Organism
SrtA
-
 Staphylococcus aureus

General Information

General Information Comment Organism
physiological function the enzyme catalyzes the covalent anchoring of surface proteins to the cell wall by linking the threonyl carboxylate of the LPXTG recognition motif to the amino group of the pentaglycine cross-bridge of the peptidoglycan Staphylococcus aureus