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Literature summary for 3.4.22.69 extracted from

  • Perera, K.D.; Rathnayake, A.D.; Liu, H.; Pedersen, N.C.; Groutas, W.C.; Chang, K.O.; Kim, Y.
    Characterization of amino acid substitutions in feline coronavirus 3C-like protease from a cat with feline infectious peritonitis treated with a protease inhibitor (2019), Vet. Microbiol., 237, 108398 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.69

Protein Variants

Protein Variants Comment Organism
A252S A252S mutation in 3CLpro confers an 1.15fold increase in the IC50 value for the inhibitor GC376 and a 1.24fold increase in the IC50 value for the inhibitor NPI52 feline coronavirus
K260N K260N mutation in 3CLpro confers an 1.05fold increase in the IC50 value for the inhibitor GC376 feline coronavirus
additional information comparison of the amino acid sequences of feline coronavirus 3CLpro from the pre-treatment ascites sample and the postmortem tissue samples of Felis catus shows amino acid changes of N25S and K260N in the kidney and N25S, A252S and K260N in the lung and spleen feline coronavirus
N25S N25S mutation in 3CLpro confers an 1.38fold increase in the IC50 value for the inhibitor GC376 and a 1.54fold increase in the IC50 value for the inhibitor NPI52 feline coronavirus
N25S/A252S/K260N N25S/A252S/K260N mutation in 3CLpro confers an 1.68fold increase in the IC50 value for the inhibitor GC376 and a 1.06fold increase in the IC50 value for the inhibitor NPI52 feline coronavirus
N25S/K260N N25S/K260N mutation in 3CLpro confers an 1.53fold increase in the IC50 value for the inhibitor GC376 and a 1.33fold increase in the IC50 value for the inhibitor NPI52 feline coronavirus

Inhibitors

Inhibitors Comment Organism Structure
GC376 peptidomimetic antiviral drug. A single amino acid change of N25S, but not A252S or K260N, leads to a statistically significant increase in the IC50 value as compared to that of wild-typwe enzyme feline coronavirus
NPI52
-
 feline coronavirus

Organism

Organism UniProt Comment Textmining
feline coronavirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O
-
 feline coronavirus Dabcyl-KTSAVLQ + SGFRKME-EDANS
-
 ?

Synonyms

Synonyms Comment Organism
3cLpro
-
 feline coronavirus
coronavirus 3C-like protease
-
 feline coronavirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at feline coronavirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at feline coronavirus