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Literature summary for 3.4.22.69 extracted from

  • Chen, S.; Zhang, J.; Hu, T.; Chen, K.; Jiang, H.; Shen, X.
    Residues on the dimer interface of SARS coronavirus 3C-like protease: dimer stability characterization and enzyme catalytic activity analysis (2008), J. Biochem., 143, 525-536.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Severe acute respiratory syndrome-related coronavirus

Protein Variants

Protein Variants Comment Organism
E14A the ratio of dimer to monomer in solution is 0.36, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
F140A the ratio of dimer to monomer in solution is 0.63, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
F3A the ratio of dimer to monomer in solution is 0.93, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
R4A the ratio of dimer to monomer in solution is 0.45, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
S10A the ratio of dimer to monomer in solution is 0.66, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
S139A the ratio of dimer to monomer in solution is 0.81, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus
S1A the ratio of dimer to monomer in solution is 1.08, compared to 1 for the wild-type enzyme Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
EDANS-VNSTLQSGLRK-(Dabcyl)-M + H2O
-
Severe acute respiratory syndrome-related coronavirus EDANS-VNSTLQ + SGLRK-(Dabcyl)-M
-
?

Subunits

Subunits Comment Organism
dimer in solution the wild type protease exhibits both forms of monomer and dimer and the amount of the monomer is almost equal to that of the dimeric form Severe acute respiratory syndrome-related coronavirus
monomer in solution the wild type protease exhibits both forms of monomer and dimer and the amount of the monomer is almost equal to that of the dimeric form Severe acute respiratory syndrome-related coronavirus