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Literature summary for 3.4.22.69 extracted from

  • Hamill, P.; Hudson, D.; Kao, R.Y.; Chow, P.; Raj, M.; Xu, H.; Richer, M.J.; Jean, F.
    Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata (2006), Biol. Chem., 387, 1063-1074.
    View publication on PubMed
Search for more literature for 3.4.22.69

Application

Application Comment Organism
analysis developing a novel red-shifted fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms Severe acute respiratory syndrome-related coronavirus

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged SARS-CoV 3CL protease expressed in Escherichia coli Severe acute respiratory syndrome-related coronavirus

Inhibitors

Inhibitors Comment Organism Structure
esculetin-4-carboxylic acid ethyl ester a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata Severe acute respiratory syndrome-related coronavirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.146
-
 o-aminobenzoyl-TSAVLQSGFRY(NO2)G
-
 Severe acute respiratory syndrome-related coronavirus

Organism

Organism UniProt Comment Textmining
Severe acute respiratory syndrome-related coronavirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged SARS-CoV 3CL protease Severe acute respiratory syndrome-related coronavirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(CAL fluor red 610)-TSAVLQSGFRK(BHQ1) + H2O
-
 Severe acute respiratory syndrome-related coronavirus (CAL fluor red 610)-TSAVLQ + SGFRK(BHQ1)
-
 ?
o-aminobenzoyl-TSAVLQSGFRY(3-NO2)G + H2O
-
 Severe acute respiratory syndrome-related coronavirus o-aminobenzoyl-TSAVLQ + SGFRY(3-NO2)G
-
 ?

Synonyms

Synonyms Comment Organism
SARS-coronavirus 3CL protease
-
 Severe acute respiratory syndrome-related coronavirus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
 o-aminobenzoyl-TSAVLQSGFRY(NO2)G
-
 Severe acute respiratory syndrome-related coronavirus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.046
-
-
 Severe acute respiratory syndrome-related coronavirus esculetin-4-carboxylic acid ethyl ester