Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | strong inhibition | Zingiber officinale | |
Co2+ | slight inhibition | Zingiber officinale | |
Cu2+ | complete inhibition | Zingiber officinale | |
Hg2+ | complete inhibition | Zingiber officinale | |
Mn2+ | slight inhibition | Zingiber officinale | |
Zn2+ | slight inhibition | Zingiber officinale |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Zingiber officinale | |
K+ | activates | Zingiber officinale | |
additional information | the purified enzyme is highly stable against numerous metal ions | Zingiber officinale | |
Na+ | activates | Zingiber officinale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33800 | - |
native PAGE | Zingiber officinale |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zingiber officinale | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 14.91fold from rhizomes by ammonium sulfate fractionation, tert-butanol precipitation at 25°C and pH 7.0 using the interfacial precipitateand the aqueous phase, and ultrafiltration, method optimization | Zingiber officinale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rhizome | - |
Zingiber officinale | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.005 | - |
purified native enzyme, substrate bovine casein, pH 7.0, 40°C | Zingiber officinale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bovine casein + H2O | - |
Zingiber officinale | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33800, SDS-PAGE | Zingiber officinale |
Synonyms | Comment | Organism |
---|---|---|
zingibain | - |
Zingiber officinale |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Zingiber officinale |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | purified native enzyme, pH 7.0, 2 h, stable | Zingiber officinale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
broad optimum at pH 6.0-7.5 | Zingiber officinale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8.5 | activity range, profile overview | Zingiber officinale |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zingiber officinale | isoelectric focussing | - |
4.38 |