Any feedback?
Literature summary for 3.4.22.66 extracted from
- Insights into the enzyme-substrate interaction in the norovirus 3C-like protease (2009), J. Biochem., 146, 509-521.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells | Chiba virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E54L | the E54L mutant protease is a substrate-specificity mutant and requires large hydrophobic amino acid residues at both P4 and P2 positions of the substrate, it cleaves the sequence 133LSFE/AP between the 3B and 3C regions of norovirus polyprotein, but does not cleaves the sequence 198ATSE/GK between the 3A and 3B | Chiba virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-Leu-Gly-Gly | - |
Chiba virus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| norovirus polyprotein + H2O | Chiba virus | - |
hydrolyzed norovirus polyprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chiba virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| GSTrap FF column chromatography | Chiba virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| norovirus polyprotein + H2O | - |
Chiba virus | hydrolyzed norovirus polyprotein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3C-like protease | - |
Chiba virus |
| 3CP | - |
Chiba virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
