Literature summary for 3.4.22.66 extracted from

Lee, C.C.; Kuo, C.J.; Ko, T.P.; Hsu, M.F.; Tsui, Y.C.; Chang, S.C.; Yang, S.; Chen, S.J.; Chen, H.C.; Hsu, M.C.; Shih, S.R.; Liang, P.H.; Wang, A.H.
Structural basis of inhibition specificities of 3C and 3C-like proteases by zinc-coordinating and peptidomimetic Compounds (2009), J. Biol. Chem., 284, 7646-7655.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Human coronavirus 229E

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 18% (w/v) PEG 6000, 10% (v/v) DMSO, 14% methyl-2,4-pentanediol, and 0.12 M Tris-HCl, pH 8.5	Human coronavirus 229E

Inhibitors

Inhibitors	Comment	Organism	Structure
TG-0203770	-	Human coronavirus 229E
TG-0204998	-	Human coronavirus 229E
TG-0205221	-	Human coronavirus 229E
TG-0205486	-	Human coronavirus 229E
zinc N-ethyl-N-phenyldithiocarbamate	-	Human coronavirus 229E

Organism

Organism	UniProt	Comment	Textmining
Human coronavirus 229E	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and HiTrap 26/10 QFF column chroamtography	Human coronavirus 229E

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dabcyl-KTSAVLQSGFRKME-EDANS + H2O	-	Human coronavirus 229E	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Human coronavirus 229E

Synonyms

Synonyms	Comment	Organism
3C-like protease	-	Human coronavirus 229E
3cLpro	-	Human coronavirus 229E

General Information

General Information	Comment	Organism
physiological function	the enzyme is required for viral polyprotein processing	Human coronavirus 229E

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Release 2023.1 (January 2023)