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Literature summary for 3.4.22.66 extracted from

  • Someya, Y.; Takeda, N.; Miyamura, T.
    Characterization of the norovirus 3C-like protease (2005), Virus Res., 110, 91-97.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
IPTG induction of gene expression Chiba virus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Chiba virus

Protein Variants

Protein Variants Comment Organism
C139A cysteine residue responsible for hydrolysis Chiba virus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 20% inhibited by 0.01 mM, 40% inhibited by 5 mM Chiba virus
Hg2+ 5 mM completely inhibits Chiba virus
K+ inhibited by 100 mM Chiba virus
methyl methanethiosulfonate almost complete inhibition Chiba virus
additional information not inhibited by Mg2+, Ca2+, 0.01 mM iodoacetate nor 0.01 mM iodoacetamide Chiba virus
N-ethylmaleimide almost complete inhibition Chiba virus
N-Phenylmaleimide almost complete inhibition Chiba virus
Na+ 83% inhibited by 100 mM Chiba virus
p-chloromercuribenzenesulfonate 27% inhibited by 0.01 mM Chiba virus
p-chloromercuribenzoic acid 82% inhibited by 0.01 mM Chiba virus
phenyl methylsulfonylfluoride 20% inhibition by 0.01 mM Chiba virus
Zn2+ 5 mM completely inhibits Chiba virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20600
-
calculated from ProHis protein Chiba virus

Organism

Organism UniProt Comment Textmining
Chiba virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chiba virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
His3Cd-GST-protein + H2O
-
Chiba virus ?
-
?

Synonyms

Synonyms Comment Organism
3C-like protease
-
Chiba virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
80% enzyme activity Chiba virus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 48
-
Chiba virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.62
-
100% enzyme activity Chiba virus

pH Range

pH Minimum pH Maximum Comment Organism
7.22 9.08
-
Chiba virus