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Literature summary for 3.4.22.63 extracted from

  • Arockiaraj, J.; Gnanam, A.J.; Muthukrishnan, D.; Pasupuleti, M.; Milton, J.; Singh, A.
    An upstream initiator caspase 10 of snakehead murrel Channa striatus, containing DED, p20 and p10 subunits: molecular cloning, gene expression and proteolytic activity (2013), Fish Shellfish Immunol., 34, 505-513.
    View publication on PubMed
Search for more literature for 3.4.22.63

Cloned(Commentary)

Cloned (Comment) Organism
gene CsCasp10, cDNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative real time PCR expression analysis, recombinant expression of enzyme fused to maltose-binding protein in Escherichia coli strain BL21(DE3) Channa striata

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information the enzyme's amino acid sequence does not have either signal peptide region or transmembrane region Channa striata
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 recombinant enzyme cleaved from maltose-binding protein-fusion protein, gel filtration Channa striata
103500
-
 1 * 61000, about, sequence calculation, mature enzyme, 1 * 103500, recombinant maltose-binding protein-fusion enzyme, SDS-PAGE Channa striata

Organism

Organism UniProt Comment Textmining
Channa striata T1RRM8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose-binding protein-fused enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography Channa striata

Source Tissue

Source Tissue Comment Organism Textmining
gill high exxpression level Channa striata
-
intestine low exxpression level Channa striata
-
kidney
-
 Channa striata
-
spleen
-
 Channa striata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0025
-
 purified recombinant enzyme, pH not specified in the publication, 37°C Channa striata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AEVD-4-nitroanilide + H2O
-
 Channa striata AEVD + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61000, about, sequence calculation, mature enzyme, 1 * 103500, recombinant maltose-binding protein-fusion enzyme, SDS-PAGE Channa striata
More the enzyme contains two death effector domains (DED) in the N-terminal at 2-77 and 87-154 and it contains caspase family p20 domain (large subunit) and caspase family p10 domain (small subunit) in the C-terminal at 299-425 and 449-536 respectively Channa striata

Synonyms

Synonyms Comment Organism
CASP10
-
 Channa striata
caspase 10
-
 Channa striata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Channa striata

pI Value

Organism Comment pI Value Maximum pI Value
Channa striata sequence calculation, mature enzyme
-
 5.2

Expression

Organism Comment Expression
Channa striata the CsCasp10 gene expression in Channa striata is upregulated in gills by fungus Aphanomyces invadans and bacteria Aeromonas hydrophila induction up

General Information

General Information Comment Organism
physiological function the enzyme is involved in immunity reactions against infection by Aphanomyces invadans and bacteria Aeromonas hydrophila Channa striata