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Literature summary for 3.4.22.63 extracted from

  • Fernandes-Alnemri, T.; Armstrong, R.C.; Krebs, J.F.; Srinivasula, S.M.; Wang, L.; Bullrich, F.; Fritz, L.C.; Trapani, J.A.; Tomaselli, K.J.; Litwack, G.; Alnemri, E.S.
    In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains (1996), Proc. Natl. Acad. Sci. USA, 93, 7464-7469.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
DEVD-aldehyde
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
DEVD-7-amido-4-methylcoumarin pH 7.5 Homo sapiens
0.15
-
YVAD-7-amido-4-methylcoumarin pH 7.5 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92851
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification Mch4 is derived from a single chain proenzym, granzyme B cleaves proMch4 at a IXXD-A processing sequence to produce mature Mch4 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CPP32 + H2O
-
Homo sapiens ?
-
?
DEVD-7-amido-4-methylcoumarin + H2O
-
Homo sapiens DEVD + 7-amino-4-methylcoumarin
-
?
pro-Mch3 + H2O cleaves the propeptide of Mch3 to generate a 33000 Da protein which is further processed to the 20000 Da band and the 12000 Da protein Homo sapiens ?
-
?
YVAD-7-amido-4-methylcoumarin + H2O
-
Homo sapiens YVAD + 7-amino-4-methylcoumarin
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000014
-
DEVD-aldehyde pH 7.5 Homo sapiens