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Literature summary for 3.4.22.62 extracted from

  • Zhao, J.F.; Sun, A.H.; Ruan, P.; Zhao, X.H.; Lu, M.Q.; Yan, J.
    Vibrio vulnificus cytolysin induces apoptosis in HUVEC, SGC-7901 and SMMC-7721 cells via caspase-9/3-dependent pathway (2009), Microb. Pathog., 46, 194-200.
    View publication on PubMed
Search for more literature for 3.4.22.62

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-3 + H2O Homo sapiens
-
 caspase-3 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HUVEC cell cells treated with 10 mg/ml recombinant Vibrio vulnificus cytolysin (rVVC) at 37°C for 4 h, cell viability assay Homo sapiens
-
SGC-7901 cell cells treated with 10 mg/ml recombinant Vibrio vulnificus cytolysin (rVVC) at 37°C for 4 h, cell viability assay Homo sapiens
-
SMMC-7721 cell cells treated with 10 mg/ml recombinant Vibrio vulnificus cytolysin (rVVC) at 37°C for 4 h, cell viability assay Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 cytotoxiciy of Vibrio vulnificus cytolysin (VVC) analyzed by treatment of cell cultures with recombinant VVC protein (rVVC), recombinant Vibrio vulnificus cytolysin (rVVC) induces apoptosis in a time- and dose-dependent manner, apoptosis induced by recombinant Vibrio vulnificus cytolysin (rVVC) mediated by caspase-9 activation rather than caspase-8 activation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-LEHD-7-amido-4-trifluoromethyl coumarin + H2O activity of caspase-9 determined after treatment of cells with 10 mg/ml recombinant Vibrio vulnificus cytolysin (rVVC) for 0.5, 1, 2, 3 and 4 h Homo sapiens acetyl-LEHD + 7-amino-4-trifluoromethyl coumarin
-
 ?
pro-caspase-3 + H2O
-
 Homo sapiens caspase-3 + ?
-
 ?
pro-caspase-3 + H2O gradual increase in caspase-3 activities in cells treated with recombinant Vibrio vulnificus cytolysin (rVVC) during 0.5-3 h incubation and a slight decrease after incubation for 4 h Homo sapiens caspase-3 + ?
-
 ?