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Literature summary for 3.4.22.62 extracted from

  • Checinska, A.; Giaccone, G.; Rodriguez, J.A.; Kruyt, F.A.; Jimenez, C.R.
    Comparative proteomics analysis of caspase-9-protein complexes in untreated and cytochrome c/dATP stimulated lysates of NSCLC cells (2009), J. Proteomics, 72, 575-585.
    View publication on PubMed
Search for more literature for 3.4.22.62

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 processed caspase-9 protein, SDS-PAGE Homo sapiens
37000
-
 processed caspase-9 protein, SDS-PAGE Homo sapiens
47000
-
 un-processed caspase-9 protein, SDS-PAGE Homo sapiens
68000
-
 observed molecular weight of caspase-9 by tandem mass spectrometry in cell lysates with and without cytochrome c/dATP treatment Homo sapiens
69200
-
 calculated molecular weight of caspase-9 by tandem mass spectrometry in cell lysates with and without cytochrome c/dATP treatment Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens acetylserotonin-O-methyltransferase-like protein identified as a candidate substrate of caspase-9, cytoskeletal proteins associated with un-processed caspase-9 are potential candidates for structural stabilization or as caspase substrates during apoptosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Source Tissue

Source Tissue Comment Organism Textmining
non-small cell lung cancer cell line human H460 NSCLC cells with and without cytochrome c/(d)ATP-induced activation of apoptosis Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 proteomic analysis of caspase-9-associated protein complexes, one-dimensional gel electrophoresis and tandem mass spectrometry, 24 differential caspase-9 interactors identified, assigned to cytoskeletal organization, cell motility, catalytic activity, transcription and apoptosis, galectin-3, swiprosin-1 and the membrane-cytoskeleton linkers ezrin/radixin/moesin identified as novel caspase-9 interactors, co-immunoprecipitation and Western blot to confirm interaction of caspase-9 with identified binding partners Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acetylserotonin-O-methyltransferase-like protein identified as a candidate substrate of caspase-9, cytoskeletal proteins associated with un-processed caspase-9 are potential candidates for structural stabilization or as caspase substrates during apoptosis Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, processed caspase-9 protein, SDS-PAGE Homo sapiens
? x * 37000, processed caspase-9 protein, SDS-PAGE Homo sapiens
? x * 47000, un-processed caspase-9 protein, SDS-PAGE Homo sapiens
? x * 68000, observed molecular weight of caspase-9 by tandem mass spectrometry in cell lysates with and without cytochrome c/dATP treatment Homo sapiens
? x * 69200, calculated molecular weight of caspase-9 by tandem mass spectrometry in cell lysates with and without cytochrome c/dATP treatment Homo sapiens