Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, recombinant protein, pcDNA3, pET28a, pEGFP vectors | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C287A | immunofluorescence staining of cells transiently transfected with vectors encoding caspase-9 point mutation | Homo sapiens |
T125A/C287A | site-directed mutagenesis, immunofluorescence staining of cells transiently transfected with vectors encoding caspase-9 double mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | DYRK1A-dependent phosphorylation of Thr125 blocked by the kinase inhibitor harmine | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | phosphorylation of caspase-9 by DYRK1A involves co-localization to the nucleus | Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | threshold for the activation of caspase-9 through basal inhibitory phosphorylation at Thr125, catalyzed by the protein kinase DYRK1A | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55211 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phophorylation of caspase-9 at the inhibitory threonine residue 125 catalyzed by the protein kinase DYRK1A | Homo sapiens |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | effect of the kinase inhibitor harmine on the phosphorylation of Thr125 and on endogenous caspase-9 | Homo sapiens | - |
U2-OS cell | transfected with vectors carrying caspase-9 point mutations and GFP-DYRK1A, immunofluorescence staining | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
caspase-9 identified as a substrate of the protein kinase DYRK1A, in vitro translation and immunoprecipitation of FLAG-DYRK1A in absence or presence of kinase inhibitors analyzed, the kinase inhibitor harmine prevents inhibitory phosphorylation of caspase-9 on threonine residue 125, depletion of DYRK1A by RNA silencing inhibits phosphorylation of caspase-9 on Thr125 in cellular assays, co-expression analysis shows interaction between DYRK1A with caspase-9 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | threshold for the activation of caspase-9 through basal inhibitory phosphorylation at Thr125, catalyzed by the protein kinase DYRK1A | Homo sapiens | ? | - |
? |