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Literature summary for 3.4.22.62 extracted from

  • Seifert, A.; Allan, L.A.; Clarke, P.R.
    DYRK1A phosphorylates caspase 9 at an inhibitory site and is potently inhibited in human cells by harmine (2008), FEBS J., 275, 6268-6280.
    View publication on PubMed
Search for more literature for 3.4.22.62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, recombinant protein, pcDNA3, pET28a, pEGFP vectors Homo sapiens

Protein Variants

Protein Variants Comment Organism
C287A immunofluorescence staining of cells transiently transfected with vectors encoding caspase-9 point mutation Homo sapiens
T125A/C287A site-directed mutagenesis, immunofluorescence staining of cells transiently transfected with vectors encoding caspase-9 double mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information DYRK1A-dependent phosphorylation of Thr125 blocked by the kinase inhibitor harmine Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus phosphorylation of caspase-9 by DYRK1A involves co-localization to the nucleus Homo sapiens 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens threshold for the activation of caspase-9 through basal inhibitory phosphorylation at Thr125, catalyzed by the protein kinase DYRK1A ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phophorylation of caspase-9 at the inhibitory threonine residue 125 catalyzed by the protein kinase DYRK1A Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell effect of the kinase inhibitor harmine on the phosphorylation of Thr125 and on endogenous caspase-9 Homo sapiens
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U2-OS cell transfected with vectors carrying caspase-9 point mutations and GFP-DYRK1A, immunofluorescence staining Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 caspase-9 identified as a substrate of the protein kinase DYRK1A, in vitro translation and immunoprecipitation of FLAG-DYRK1A in absence or presence of kinase inhibitors analyzed, the kinase inhibitor harmine prevents inhibitory phosphorylation of caspase-9 on threonine residue 125, depletion of DYRK1A by RNA silencing inhibits phosphorylation of caspase-9 on Thr125 in cellular assays, co-expression analysis shows interaction between DYRK1A with caspase-9 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information threshold for the activation of caspase-9 through basal inhibitory phosphorylation at Thr125, catalyzed by the protein kinase DYRK1A Homo sapiens ?
-
 ?