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Literature summary for 3.4.22.62 extracted from

  • Arnault, E.; Tosca, L.; Courtot, A.M.; Doussau, M.; Pesty, A.; Finaz, C.; Allemand, I.; Lefevre, B.
    Caspase-2(L), caspase-9, and caspase-3 during in vitro maturation and fragmentation of the mouse oocyte (2008), Dev. Dyn., 237, 3892-3903.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzyloxycarbonyl-VAD-fluoromethylketone i.e. Z-VAD-FMK, in vitro oocyte meiosis and fragmentation analyzed by treatment with concentrations of 50 and 100 microM, transition from metaphase I to metaphase II accelerated in oocytes, comparative treatment with the apoptotic inducer doxorubicin (DXR) Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm intracellular localization of caspase-9 in germinal vesicle stage oocytes analyzed, faint and homogeneous distribution, fluorescent staining Mus musculus 5737
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nucleus intracellular localization of caspase-9 in germinal vesicle stage oocytes analyzed, distribution related to chromatin configuration Mus musculus 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-2L + H2O Mus musculus mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms caspase-2L + ?
-
?
pro-caspase-3 + H2O Mus musculus mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms caspase-3 + ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R0T0 6-week-old female CD1 mice
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Source Tissue

Source Tissue Comment Organism Textmining
oocyte immature, germinal vesicle stage oocytes, GV-oocytes Mus musculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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analysis of oocytes from prophase I onwards, inhibition of caspase activity accelerates transition from metaphase I to metaphase II, caspase-9 and caspase-3 detected along the meiotic spindle, similar amounts of cleaved caspases in healthy and fragmented oocytes, caspase inhibition does not prevent doxorubicin-induced apoptosis, cleaved caspases probably dispensable for final oocyte death, potential involvement in the regulation of oocyte maturation Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-caspase-2L + H2O mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms Mus musculus caspase-2L + ?
-
?
pro-caspase-2L + H2O mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms Mus musculus caspase-2L + ?
-
?
pro-caspase-3 + H2O mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms Mus musculus caspase-3 + ?
-
?