Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-VAD-fluoromethylketone | i.e. Z-VAD-FMK, in vitro oocyte meiosis and fragmentation analyzed by treatment with concentrations of 50 and 100 microM, transition from metaphase I to metaphase II accelerated in oocytes, comparative treatment with the apoptotic inducer doxorubicin (DXR) | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | intracellular localization of caspase-9 in germinal vesicle stage oocytes analyzed, faint and homogeneous distribution, fluorescent staining | Mus musculus | 5737 | - |
nucleus | intracellular localization of caspase-9 in germinal vesicle stage oocytes analyzed, distribution related to chromatin configuration | Mus musculus | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-2L + H2O | Mus musculus | mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms | caspase-2L + ? | - |
? | |
pro-caspase-3 + H2O | Mus musculus | mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms | caspase-3 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9R0T0 | 6-week-old female CD1 mice | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | immature, germinal vesicle stage oocytes, GV-oocytes | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of oocytes from prophase I onwards, inhibition of caspase activity accelerates transition from metaphase I to metaphase II, caspase-9 and caspase-3 detected along the meiotic spindle, similar amounts of cleaved caspases in healthy and fragmented oocytes, caspase inhibition does not prevent doxorubicin-induced apoptosis, cleaved caspases probably dispensable for final oocyte death, potential involvement in the regulation of oocyte maturation | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-2L + H2O | mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms | Mus musculus | caspase-2L + ? | - |
? | |
pro-caspase-2L + H2O | mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms | Mus musculus | caspase-2L + ? | - |
? | |
pro-caspase-3 + H2O | mechanisms of apoptotic pathways controlling fragmentation of unfertilized ovulated oocyte, caspase-9, caspase-3 and caspase-2L (long isoform) transcripts and proteins present in oocytes during ealy stages of meiosis, zymogen and cleaved forms | Mus musculus | caspase-3 + ? | - |
? |