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Literature summary for 3.4.22.62 extracted from

  • Zuo, Y.; Xiang, B.; Yang, J.; Sun, X.; Wang, Y.; Cang, H.; Yi, J.
    Oxidative modification of caspase-9 facilitates its activation via disulfide-mediated interaction with APAF-1 (2009), Cell Res., 19, 449-457.
    View publication on PubMed
Search for more literature for 3.4.22.62

Activating Compound

Activating Compound Comment Organism Structure
APAF-1 interaction with APAF-1 promotes auto-cleavage and activation of caspase-9 Homo sapiens

Application

Application Comment Organism
pharmacology studies on molecular mechanisms of caspase-9 activation mediated by reactive oxygen species (ROS) Homo sapiens

Protein Variants

Protein Variants Comment Organism
C403S point mutation at C403 of caspase-9 impairs its activation mediated by H2O2, interaction with APAF-1 diminished through the abolition of disulfide formation, weaker association between cytochrome c and the C403S mutant than that between cytochrome c and wild-type caspase-9 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens mechanisms of caspase-9 activation mediated by reactive oxygen species (ROS), caspase-9 activation facilitated by cellular oxidative state ?
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Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
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Source Tissue

Source Tissue Comment Organism Textmining
oral squamous cell carcinoma cell wild-type and SOD-antisense Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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 hydrogen peroxide application leads to activation of caspase-9 and apoptosis, promoted interaction between caspase-9 and apoptotic protease-activating factor 1 (APAF-1), thiol-oxidant diamide reveals auto-cleavage of caspase-9 and interaction of caspase-9 and APAF-1, formation of disulfide-linked complexes facilitated, in vitro analysis of a mitochondria-free system, point mutation at C403 of caspase-9 impairs caspase-9 activation induced by H2O2, interaction with APAF-1 diminished by impaired disulfide formation, mutant analysis shows that oxidative modification of caspase-9 contributes to the formation of the apoptosome under oxidative stress Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information mechanisms of caspase-9 activation mediated by reactive oxygen species (ROS), caspase-9 activation facilitated by cellular oxidative state Homo sapiens ?
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 ?
additional information apoptosome formation and caspase-9 activation under oxidative stress, oxidative modification of caspase-9 mediates interaction with APAF-1 and promotes its auto-cleavage and activation Homo sapiens ?
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 ?