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Literature summary for 3.4.22.62 extracted from

  • Eeva, J.; Nuutinen, U.; Ropponen, A.; Maettoe, M.; Eray, M.; Pellinen, R.; Wahlfors, J.; Pelkonen, J.
    The involvement of mitochondria and the caspase-9 activation pathway in rituximab-induced apoptosis in FL cells (2009), Apoptosis, 14, 687-698.
    View publication on PubMed
Search for more literature for 3.4.22.62

Application

Application Comment Organism
pharmacology rituximab-induced apoptosis highly dependent on caspase-9 activation, regulated by Bcl-xL expression Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
caspase-9 inactivated by mutation at the catalytic domain, generation of dominant negative caspase-9 overexpressing cell lines, expression in Escherichia coli, follicular lymphoma cells transformed with the lentiviral vector pWPI-IRES-GFP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information rituximab-induced apoptosis involved in the activation of caspase-9 can be blocked by Bcl-xL overexpression Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 full-length caspase-9, Western blot analysis Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-3 + H2O Homo sapiens signalling pathways of CD20-induced apoptosis analyzed by using the chimeric mouse-human anti-CD20 antibody rituximab, activation of caspase-9 essential for rituximab-mediated apoptosis, mitochondrial role in rituximab-induced apoptosis, overexpression of caspase-9 inhibits the rituximab-induced activation of caspase-3 caspase-3 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
FL cell cell line HF1A3, wild-type, dominant negative caspase-9 overexpressing Homo sapiens
-
follicular lymphoma cell cell line HF1A3, wild-type, dominant negative caspase-9 overexpressing Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 contribution of the death receptor and mitochondrial (intrinsic) pathways to apoptosis in follicular lymphoma cells induced by the chimeric mouse-human anti-CD20 antibody rituximab, time course of caspase activation, overexpression of caspase-9 inhibits the rituximab-induced activation of effector proteases (caspase-3, caspase-6 and caspase-7), rituximab-induced release of cytochrome c and collapse of mitochondrial membrane potential regulated by caspase-9 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-caspase-3 + H2O signalling pathways of CD20-induced apoptosis analyzed by using the chimeric mouse-human anti-CD20 antibody rituximab, activation of caspase-9 essential for rituximab-mediated apoptosis, mitochondrial role in rituximab-induced apoptosis, overexpression of caspase-9 inhibits the rituximab-induced activation of caspase-3 Homo sapiens caspase-3 + ?
-
 ?