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Literature summary for 3.4.22.61 extracted from

  • Edelmann, B.; Bertsch, U.; Tchikov, V.; Winoto-Morbach, S.; Perrotta, C.; Jakob, M.; Adam-Klages, S.; Kabelitz, D.; Schuetze, S.
    Caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes (2011), EMBO J., 30, 379-394.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.61

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information active caspase-8 colocalizes with internalized TNF-R1 receptosomes, direct binding of caspase-8 to TNF-R1 on internalized receptosomes, molecular components of a potential signalling cascade from TNF-R1 to A-SMase via caspase-8 actually localize in the same subcellular compartment Homo sapiens
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 1 * 41000-43000 + 1 * 18000, mature caspase-8, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens caspase-8 is involved in activation of acid sphingomyelinase induced by TNF, but caspase-8 does not display firm association neither with caspase-7 nor with A-SMase, overview. Direct binding of caspase-8 to TNF-R1 on internalized receptosomes ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage of pro-caspase-8, 54/52 kDa, to the active form of 18 kDa in TNF receptosomes Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
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 Homo sapiens
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JURKAT cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information caspase-8 is involved in activation of acid sphingomyelinase induced by TNF, but caspase-8 does not display firm association neither with caspase-7 nor with A-SMase, overview. Direct binding of caspase-8 to TNF-R1 on internalized receptosomes Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 41000-43000 + 1 * 18000, mature caspase-8, SDS-PAGE Homo sapiens
monomer 1 * 52000-54000, procaspase-8, SDS-PAGE Homo sapiens

General Information

General Information Comment Organism
malfunction no change in A-SMase activity in caspase-8-deficient cells. Retransfection of caspase-8-deficient Jurkat cells with an expression plasmid for caspase-8 restores the ability of the transfected cells to respond to TNF stimulation by increased A-SMase activity. Caspase-8-deficient Jurkat cells are almost completely resistant to TNF/CHX treatment Homo sapiens
additional information active caspase-8 colocalizes with internalized TNF-R1 receptosomes Homo sapiens
physiological function caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. TNF-induced A-SMase activation strictly depends on functional caspase-8 and caspase-7 expression Homo sapiens