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Literature summary for 3.4.22.60 extracted from

  • Hill, M.E.; MacPherson, D.J.; Wu, P.; Julien, O.; Wells, J.A.; Hardy, J.A.
    Reprogramming caspase-7 specificity by regio-specific mutations and selection provides alternate solutions for substrate recognition (2016), ACS Chem. Biol., 11, 1603-1612 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.60

Protein Variants

Protein Variants Comment Organism
additional information mutational analysis to introduce the specificity of caspase-6 into caspase-7. Substitution of substrate-contacting residues from caspase-6 into caspase-7 is ineffective, yielding an inactive enzyme, whereas saturation mutagenesis at these positions and selection by directed evolution produces active caspases. The structures of the evolved-specificity caspase-7 reveal alternate binding modes for the substrate, including reorganization of an active site loop Homo sapiens
Y230V/W232Y/S234V/Q276D variant closely micks the substrate specificty of caspase 6, but does not cleave lamin C, a known caspase-6-specific substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0054
-
 Ac-VEID-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.041
-
 Ac-DEVD-7-amido-4-methylcoumarin wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.041
-
 Ac-DEVD-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.2
-
 Ac-VEID-7-amido-4-methylcoumarin wild-type, value above 0.2 mM, pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P55210
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DEVD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-DEVD + 7-amino-4-methylcoumarin
-
 ?
Ac-VEID-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ac-VEID + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
Casp7
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Ac-VEID-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.9
-
 Ac-DEVD-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
2.4
-
 Ac-DEVD-7-amido-4-methylcoumarin wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
 Ac-DEVD-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
30
-
 Ac-VEID-7-amido-4-methylcoumarin Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication Homo sapiens
60
-
 Ac-DEVD-7-amido-4-methylcoumarin wild-type, pH not specified in the publication, temperature not specified in the publication Homo sapiens