Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockdown by caspase-7 siRNA | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Z-DEVD-fluoromethylketone | a caspase-3 and caspase-7 inhibitor | Homo sapiens | |
Z-VAD-fluoromethylketone | a pan-caspase inhibitor | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Kaposi sarcoma-associated herpesvirus ORF57 + H2O | Homo sapiens | a viral early protein essential for KSHV multiplication. Cleavage of wild-type or mutant D33A ORF57 by valproate-induced BCBL-1 cell extracts containing active caspases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
B-lymphocyte | B cells derived from cavity-based B cell lymphoma | Homo sapiens | - |
B-lymphoma cell | B cells derived from cavity-based B cell lymphoma | Homo sapiens | - |
BCBL-1 cell | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Kaposi sarcoma-associated herpesvirus ORF57 + H2O | a viral early protein essential for KSHV multiplication. Cleavage of wild-type or mutant D33A ORF57 by valproate-induced BCBL-1 cell extracts containing active caspases | Homo sapiens | ? | - |
? | |
Kaposi sarcoma-associated herpesvirus ORF57 + H2O | a viral early protein essential for KSHV multiplication, caspase-7 cleavage site is 30DETD33 | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of caspase-7 from the cytoplasmic extracts of valproate-induced BCBL-1 cells reduces the cleavage of ORF57, and inhibition of caspase-7 activity promotes the expression of ORF59 | Homo sapiens |
physiological function | caspase-7 cleavage of Kaposi sarcoma-associated herpesvirus ORF57 in the cytoplasm confers a cellular function against viral lytic gene expression. But only initiator caspase-8, EC 3.4.22.61, is triggered in BCBL-1 cells with lytic KSHV induction by valproate, along with cleavage of expressed ORF57. Caspase-7 is an essential caspase in the cleavage of KSHV ORF57 and plays a key role in ORF57 cleavage in BCBL-1 cells during KSHV lytic infection | Homo sapiens |