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Literature summary for 3.4.22.60 extracted from

  • Lee, Y.M.; Kang, H.J.; Jang, M.; Kim, M.; Bae, K.H.; Chung, S.J.
    Large-scale expression in Escherichia coli and efficient purification of precursor and active caspase-7 by introduction of thrombin cleavage sites (2010), Protein Expr. Purif., 69, 29-33.
    View publication on PubMed
Search for more literature for 3.4.22.60

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D198A additionally, amino acid residues 20-25 are replaced with a thrombin cleavage site (LVPRGS) and a thrombin cleavage site (LVPRGS) is inserted between Asp-203 and Thr-204 Homo sapiens
additional information caspase cleavage site is substituted and a thrombin cleavage site inserted: amino acid residues 20-25 are replaced with a thrombin cleavage site (LVPRGS) and a thrombin cleavage site (LVPRGS) is inserted between Asp-203 and Thr-204 Homo sapiens
additional information caspase cleavage sites are substituted: amino acid residues 20-25 and 195-200 are replaced with thrombin cleavage sites (LVPRGS) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0848
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin Homo sapiens
0.111
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide wild-type protein Homo sapiens
0.1193
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 Homo sapiens
0.415
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin Homo sapiens
0.9365
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Co2+) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O
-
 Homo sapiens N-acetyl-Asp-Glu-Val-Asp + p-nitroaniline
-
 ?
poly(ADP-ribose)polymerase + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
caspase-7
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 Homo sapiens
0.1
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 Homo sapiens
1.9
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin Homo sapiens
3.6
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide wid-type protein Homo sapiens
4.4
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.009
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200 Homo sapiens
0.83
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203 Homo sapiens
4.7
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and 195-200, processed with thrombin Homo sapiens
33.3
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide wild-type protein Homo sapiens
51.7
-
 N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide mutant protein with thrombin cleavage sites (LVPRGS) at residues 20-25 and inserted after Asp-203, processed with thrombin Homo sapiens