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Literature summary for 3.4.22.60 extracted from

  • Jang, M.; Park, B.C.; Kang, S.; Lee, d.o..H.; Cho, S.; Lee, S.C.; Bae, K.H.; Park, S.G.
    Mining of caspase-7 substrates using a degradomic approach (2008), Mol. Cells, 26, 152-157.
    View publication on PubMed
Search for more literature for 3.4.22.60

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminally His-tagged caspase-7 in Escherichia coli strain BL21 (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
carbobenzyloxy-DEVD-fluoromethyl ketone blocks activation of caspase-7 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
actin-related protein 2/3 complex subunit 3 + H2O Homo sapiens
-
 ?
-
 ?
far upstream element binding protein 1 + H2O Homo sapiens
-
 ?
-
 ?
FK506-binding protein 4 + H2O Homo sapiens
-
 ?
-
 ?
fructose-bisphosphate aldolase A + H2O Homo sapiens
-
 ?
-
 ?
gamma-actin + H2O Homo sapiens
-
 ?
-
 ?
lamin B2 + H2O Homo sapiens
-
 ?
-
 ?
additional information Homo sapiens functions of caspase-7 during apoptosis, overview ?
-
 ?
nucleoside diphosphate kinase B + H2O Homo sapiens
-
 ?
-
 ?
proteasome subunit alpha type-2 + H2O Homo sapiens
-
 ?
-
 ?
septin 5 + H2O Homo sapiens i.e. peanut-like protein 1 ?
-
 ?
serine/threonine-protein kinase PAK2 + H2O Homo sapiens
-
 ?
-
 ?
thioredoxin peroxidase 1 + H2O Homo sapiens
-
 ?
-
 ?
valosin-containing protein + H2O Homo sapiens cleavage by caspase-7 during apoptosis ?
-
 ?
vimentin + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the procaspase-7 is cleaved to active enzyme by eliminating the prodomain, blocked by inhibitor carbobenzyloxy-DEVD-fluoromethyl ketone Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged caspase-7 from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-DEVD-4-nitroanilide + H2O
-
 Homo sapiens Ac-DEVD + 4-nitroaniline
-
 ?
actin-related protein 2/3 complex subunit 3 + H2O
-
 Homo sapiens ?
-
 ?
far upstream element binding protein 1 + H2O
-
 Homo sapiens ?
-
 ?
FK506-binding protein 4 + H2O
-
 Homo sapiens ?
-
 ?
fructose-bisphosphate aldolase A + H2O
-
 Homo sapiens ?
-
 ?
gamma-actin + H2O
-
 Homo sapiens ?
-
 ?
lamin B2 + H2O
-
 Homo sapiens ?
-
 ?
additional information functions of caspase-7 during apoptosis, overview Homo sapiens ?
-
 ?
additional information caspase-7, like caspase-3, shows substrate specificity with special preference for the DEVD motif, peptide products, overview Homo sapiens ?
-
 ?
nucleoside diphosphate kinase B + H2O
-
 Homo sapiens ?
-
 ?
proteasome subunit alpha type-2 + H2O
-
 Homo sapiens ?
-
 ?
septin 5 + H2O i.e. peanut-like protein 1 Homo sapiens ?
-
 ?
serine/threonine-protein kinase PAK2 + H2O
-
 Homo sapiens ?
-
 ?
thioredoxin peroxidase 1 + H2O
-
 Homo sapiens ?
-
 ?
valosin-containing protein + H2O cleavage by caspase-7 during apoptosis Homo sapiens ?
-
 ?
valosin-containing protein + H2O i.e. VCP or transitional endoplasmic reticulum ATPase, recombinant His-tagged substrate expressed in MCF-7 cells, cleavage at both DELD307 and DELD580, no activity with substrate mutant D307A/D580A Homo sapiens ?
-
 ?
vimentin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer caspase-7 domain organization comprising large and small subunit, and the prodomain, overview Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens