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Literature summary for 3.4.22.60 extracted from

  • Li, I.T.; Pham, E.; Chiang, J.J.; Truong, K.
    FRET evidence that an isoform of caspase-7 binds but does not cleave its substrate (2008), Biochem. Biophys. Res. Commun., 373, 325-329.
    View publication on PubMed
Search for more literature for 3.4.22.60

Cloned(Commentary)

Cloned (Comment) Organism
two truncated mutant isozymes 24casp7 and 57casp7 are cloned from a skeletal muscle cDNA library, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of two truncated mutant isozymes 24casp7 and 57casp7, which lack the first 24 and 57 amino acid residues, respectively, and thus the cleavage site at Asp23 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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 additional information binding between the mutant 24casp7 isozyme and the vDEVDc biosensor occurs exponentially Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
caspase-7 biosensor vDEVDc + H2O Homo sapiens wild-type caspase-7 binds to caspase-7 biosensor vDEVDc, but does not cleave it, while an mutant isozymes of caspase-7 with the 24 or 56 N-terminal residues truncated, i.e. 24casp7 and 57casp7, respectively, cleave vDEVDc at the recognition sequence ?
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 ?
additional information Homo sapiens caspase-7 is an executioner protease in cellular apoptosis ?
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification caspase-7 possesses two cleavage sites after Asp23 and Asp198 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caspase-7 biosensor vDEVDc + H2O wild-type caspase-7 binds to caspase-7 biosensor vDEVDc, but does not cleave it, while an mutant isozymes of caspase-7 with the 24 or 56 N-terminal residues truncated, i.e. 24casp7 and 57casp7, respectively, cleave vDEVDc at the recognition sequence Homo sapiens ?
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 ?
caspase-7 biosensor vDEVDc + H2O wild-type caspase-7 binds to caspase-7 biosensor vDEVDc, but does not cleave it, while an mutant isozymes of caspase-7 with the 24 or 56 N-terminal residues truncated, i.e. 24casp7 and 57casp7, respectively, cleave vDEVDc at the recognition sequence. Computational modeling of the vDEVDc biosensor, overview Homo sapiens ?
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 ?
additional information caspase-7 is an executioner protease in cellular apoptosis Homo sapiens ?
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additional information no activity of mutant 57casp7 isozyme with control biosensor v4Gc Homo sapiens ?
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 ?